Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction.

BMC Veterinary Research
M RiihimäkiJ Pringle

Abstract

The aim of this study was to evaluate the contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of cytokines of innate immunity IL-6, IL-10 and TNF-alpha, in horses with Recurrent Airway Obstruction (RAO) during exacerbation and in remission. Despite marked clinical and physiologic alterations between exacerbation and after remission in the RAO horses no differences were detected in either cytokine mRNA or protein levels. Moreover, the expression of investigated cytokines in RAO horses on pasture did not differ from controls. In comparing real-time PCR analysis to results of immunohistochemistry only IL-10 mRNA and protein levels in RAO horses on pasture were significantly correlated (rs = 0.893, p = 0.007). Curiously, in controls examined on pasture the TNF-alpha protein level was positively correlated to IL-10 mRNA expression (rs = 0.967, p = 0.007) and negatively correlated to IL-6 mRNA expression (rs = -0.971, p = 0.001). Given the complementary relationship of assessing cytokines directly by immunohistochemistry, or indirectly by PCR to mRNA, the lack of significant changes in either mRNA or protein levels of IL-6, IL-10 or TNF-alpha mRNA in RAO horses in exacerbation sugges...Continue Reading

References

Nov 1, 1994·American Journal of Respiratory and Critical Care Medicine·P H HowarthS T Holgate
Sep 1, 1993·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K M BrittenW R Roche
Jan 1, 1996·American Journal of Respiratory and Critical Care Medicine·Z WangK Larsson
Aug 12, 1998·American Journal of Respiratory and Critical Care Medicine·K J HaleyJ M Drazen
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·S T HolgateD Davies
Nov 1, 2000·Metabolic Engineering·L You, J Yin
Apr 12, 2002·Veterinary Immunology and Immunopathology·Steeve GiguèreMarco Franchini
Oct 3, 2003·Veterinary Immunology and Immunopathology·Dorothy M AinsworthDouglas F Antczak
Oct 14, 2003·American Journal of Respiratory and Critical Care Medicine·Peter JefferyUNKNOWN Endobronchial Biopsy Workshop
Jan 8, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Darryl A Knight, Stephen T Holgate
Feb 12, 2005·Journal of Applied Physiology·Michael S DavisKatherine K Williamson
Mar 8, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jamshid PourazarThomas Sandström
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Hajime Takizawa
Sep 15, 2005·The Journal of Allergy and Clinical Immunology·Carmela PepeQutayba Hamid
Feb 25, 2006·Journal of Veterinary Internal Medicine·Tamarinde T J M LaanJohanna Fink-Gremmels
Jun 7, 2006·Veterinary Immunology and Immunopathology·Laurent L CouëtilPierre Lekeux
Dec 13, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Annerose BerndtN Edward Robinson
Apr 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J L BrownS J Wilson

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Citations

May 18, 2010·Veterinary Research Communications·M PietraA Peli
Aug 28, 2013·Veterinary Immunology and Immunopathology·Anna E KaragianniScott R Pirie
Jul 19, 2017·Veterinary Pathology·Maria P PasoliniOrlando Paciello
Oct 30, 2013·Equine Veterinary Journal·R S Pirie
Dec 13, 2016·BMC Veterinary Research·Ann Kristin BartonHeidrun Gehlen
Oct 11, 2019·Current Allergy and Asthma Reports·M Katie SheatsJill A Poole
Oct 22, 2013·Veterinary Immunology and Immunopathology·C L SchnabelJ-M V Cavalleri

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Methods Mentioned

BETA
bronchoalveolar
biopsies
PCR
biopsy
sedation
bronchoalveolar lavage

Software Mentioned

Leica
MINITAB

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