Epithelial mesenchymal transition in smokers: large versus small airways and relation to airflow obstruction

International Journal of Chronic Obstructive Pulmonary Disease
Malik Quasir MahmoodEugene Haydn Walters

Abstract

Small airway fibrosis is the main contributor in airflow obstruction in chronic obstructive pulmonary disease. Epithelial mesenchymal transition (EMT) has been implicated in this process, and in large airways, is associated with angiogenesis, ie, Type-3, which is classically promalignant. In this study we have investigated whether EMT biomarkers are expressed in small airways compared to large airways in subjects with chronic airflow limitation (CAL) and what type of EMT is present on the basis of vascularity. We evaluated epithelial activation, reticular basement membrane fragmentation (core structural EMT marker) and EMT-related mesenchymal biomarkers in small and large airways from resected lung tissue from 18 lung cancer patients with CAL and 9 normal controls. Tissues were immunostained for epidermal growth factor receptor (EGFR; epithelial activation marker), vimentin (mesenchymal marker), and S100A4 (fibroblast epitope). Type-IV collagen was stained to demonstrate vessels. There was increased expression of EMT-related markers in CAL small airways compared to controls: EGFR (P<0.001), vimentin (P<0.001), S100A4 (P<0.001), and fragmentation (P<0.001), but this was less than that in large airways. Notably, there was no hype...Continue Reading

Citations

Mar 10, 2017·Respiratory Investigation·Sukhwinder Singh Sohal
Mar 23, 2017·Respirology : Official Journal of the Asian Pacific Society of Respirology·Mathew S EapenSukhwinder S Sohal
Jun 25, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mohit Kumar JollySukhwinder Singh Sohal
Mar 27, 2018·Human & Experimental Toxicology·J A Caruso, P M Stemmer
Mar 28, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tianwen LaiChao Cao
Sep 12, 2016·Respirology : Official Journal of the Asian Pacific Society of Respirology·Malik Q MahmoodEugene H Walters
May 22, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Mathew Suji EapenSukhwinder Singh Sohal
Nov 20, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mathew Suji EapenSukhwinder Singh Sohal
May 7, 2019·Current Protocols in Toxicology·Geraldine AedoJosé T Egaña
Mar 7, 2020·Expert Review of Molecular Diagnostics·Osnat Shtraichman, Joshua M Diamond
Jul 27, 2017·Expert Review of Respiratory Medicine·Mathew Suji EapenSukhwinder Singh Sohal
Apr 20, 2018·Biological Reviews of the Cambridge Philosophical Society·Giuseppe BronteLucio Crinò
Jul 13, 2019·The European Respiratory Journal·Wenying LuSukhwinder Singh Sohal
Jun 1, 2018·Mediators of Inflammation·Virginia De RoseCatherine M Greene
Jun 24, 2017·American Journal of Respiratory and Critical Care Medicine·Sukhwinder Singh SohalEugene Haydn Walters
Oct 15, 2020·European Journal of Clinical Investigation·Ping LinZongan Liang
Oct 29, 2020·Signal Transduction and Targeted Therapy·Cuixue WangHai Tian
Jan 19, 2020·Chest·Darryl A KnightMichael Schuliga
May 28, 2021·European Respiratory Review : an Official Journal of the European Respiratory Society·E Haydn WaltersChris Ward
Jul 13, 2021·Frontiers in Physiology·François M CarlierCharles Pilette

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Methods Mentioned

BETA
biopsies
Cytomation

Software Mentioned

Image Pro
SPSS

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