Epithelial permeability is not increased in rats following small bowel resection

The Journal of Surgical Research
D P O'BrienBrad W Warner

Abstract

Increased intestinal permeability and translocation of bacteria and/or bacterial products may cause infection and liver dysfunction in patients with the short bowel syndrome. In previous studies, serum from mice undergoing small bowel resection (SBR) enhanced growth of cultured rat intestinal epithelial cells (RIEC-6), implicating a role for a serum factor(s) in the enterocyte response to SBR. These experiments tested the hypothesis that epithelial cell permeability is increased following SBR. Male Sprague-Dawley rats underwent a 75% SBR or sham operation. Intestinal permeability in the remnant ileum was determined by Ussing chambers on Postoperative Day (POD) 3. Additionally, serum was collected on POD 1, 3, and 7 and mesenteric lymph was harvested on POD 3. Once confluent, RIEC-6 cells were incubated for 3 days in media supplemented with 10% fetal bovine serum (FBS; control), 1% FBS, 1% FBS plus 9% Sham serum, or 1% FBS plus 9% SBR serum or exposed to media with varied concentrations of SBR or Sham lymph. Monolayer permeability was determined by measuring the passage of dextran-rhodamine. Intestinal permeability was reduced in rats undergoing SBR. Sham serum-treated monolayers demonstrated the greatest permeability. Incubatio...Continue Reading

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Citations

Dec 20, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Junqiang TianThomas R Ziegler
Mar 8, 2013·Journal of Tissue Engineering and Regenerative Medicine·Yasuhiro TorashimaTracy C Grikscheit
Sep 6, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Cindy C TaiSaverio Bellusci
Apr 27, 2002·American Journal of Surgery·Lindsey A NelsonBrad W Warner
Feb 14, 2006·Gastroenterology·Eamonn M M Quigley, Rodrigo Quera
Mar 8, 2014·Acta Cirúrgica Brasileira·Aldo Cunha MedeirosMarília Daniela Ferreira Carvalho
Aug 19, 2006·Inflammatory Bowel Diseases·Anton J KroesenHeinz J Buhr
Nov 14, 2001·Current Opinion in Clinical Nutrition and Metabolic Care·H Schmidt, R Martindale

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