PMID: 6540264Jul 1, 1984Paper

Epithelial structure revealed by chemical dissection and unembedded electron microscopy

The Journal of Cell Biology
E G FeyS Penman

Abstract

Cytoskeletal structures obtained after extraction of Madin-Darby canine kidney epithelial cell monolayers with Triton X-100 were examined in transmission electron micrographs of cell whole mounts and unembedded thick sections. The cytoskeleton, an ordered structure consisting of a peripheral plasma lamina, a complex network of filaments, and chromatin-containing nuclei, was revealed after extraction of intact cells with a nearly physiological buffer containing Triton X-100. The cytoskeleton was further fractionated by extraction with (NH4)2SO4, which left a structure enriched in intermediate filaments and desmosomes around the nuclei. A further digestion with nuclease and elution with (NH4)2SO4 removed the chromatin. The stable structure that remained after this procedure retained much of the epithelial morphology and contained essentially all of the cytokeratin filaments and desmosomes and the chromatin-depleted nuclear matrices. This structural network may serve as a scaffold for epithelial organization. The cytoskeleton and the underlying nuclear matrix intermediate filament scaffold, when examined in both conventional embedded thin sections and in unembedded whole mounts and thick sections, showed the retention of many of t...Continue Reading

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Citations

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A E GoldmanR D Goldman
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B WagnerS Penman
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M KellermayerC F Hazlewood
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J P BidwellG S Stein
Jul 1, 1984·The Journal of Cell Biology·M E Stearns
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman
Apr 1, 1994·Journal of Cellular Biochemistry·J P BidwellJ B Lian
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Jan 1, 1989·Cell Motility and the Cytoskeleton·M G Price, R H Gomer
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Jul 18, 2001·The Journal of Clinical Investigation·T TakedaM G Farquhar
Jan 24, 1998·Journal of Cellular Biochemistry·G QuA R Strauch
Oct 17, 2018·Cell Death Discovery·Arada VinaiphatVisith Thongboonkerd

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