Epithelial-to-mesenchymal transition of A549 lung cancer cells exposed to electronic cigarettes

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Atena ZahediPrue Talbot

Abstract

Epithelial-to-mesenchymal transition (EMT) is the initial step enabling the metastasis of cancer cells, which often leads to death. Although smoking is a major risk factor for lung cancer, there is still widespread use of conventional cigarettes. Recently, the tobacco industry has been transformed by the introduction of electronic cigarettes (ECs), which have lower levels of carcinogens and may provide a safer alternative. Here, we investigate the ability of EC liquids and aerosols to induce an EMT in A549 lung cancer cells. Human adenocarcinoma alveolar basal epithelial cells (A549) were exposed to EC liquids and aerosols from a popular product for 3-8 days. Live cell imaging, EMT biomarker analysis, and machine learning/image processing algorithms were used to characterize changes associated with EMT. Long-term exposure of A549 cells to menthol or tobacco-flavored EC liquids or aerosols induced an EMT that was characterized by acquisition of a fibroblast-like morphology, loss of cell-to-cell junctions, internalization of E-cadherin, increased motility, and upregulation of other EMT markers. The EMT was concurrent with plasma membrane to nuclear translocation of active β-catenin. This is the first known study to show an EMT of...Continue Reading

References

Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Oct 11, 2008·International Journal of Cancer. Journal International Du Cancer·Piyali DasguptaSrikumar Chellappan
May 6, 2010·BMC Public Health·Jean-François Etter
Nov 26, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Emilija VeljkovicWanjiang Han
Jun 7, 2011·Current Biology : CB·Albert B Reynolds
Apr 10, 2012·Seminars in Cancer Biology·Daniela SpanoMassimo Zollo
Jul 24, 2012·Journal of Pharmacological and Toxicological Methods·R Z BeharP Talbot
Sep 20, 2012·Reproductive Toxicology·Vasundhra BahlPrue Talbot
Dec 4, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weifeng ZouPixin Ran
Mar 8, 2013·Tobacco Control·Maciej Lukasz GoniewiczNeal Benowitz
Feb 12, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R Z BeharP Talbot
May 16, 2014·Nature Reviews. Cancer·Sergei A Grando
Feb 1, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kieran BruneVenkataramana K Sidhaye
Feb 11, 2015·PloS One·Rachel Z BeharPrue Talbot
Apr 9, 2015·Clinical and Translational Medicine·Sarah HeerbothSibaji Sarkar
Jan 15, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Peter GoldstrawUNKNOWN International Association for the Study of Lung Cancer Staging and Prognostic Factors Committee Advisory Boards and Particip
Mar 29, 2016·Cancer Metastasis Reviews·Helmut H Popper
Jun 28, 2016·Biochemical and Biophysical Research Communications·Chad A LernerIrfan Rahman
Dec 3, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·D ThorneM Gaca
Feb 12, 2017·Cell·Arthur W LambertRobert A Weinberg
May 19, 2017·Scientific Reports·Donatella CanistroMoreno Paolini
Dec 2, 2017·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Stella TommasiAhmad Besaratinia
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hyun-Wook LeeMoon-Shong Tang

❮ Previous
Next ❯

Citations

May 31, 2019·Clinical Otolaryngology : Official Journal of ENT-UK ; Official Journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·Susanne FlachJaiganesh Manickavasagam
Nov 9, 2019·Internal and Emergency Medicine·Kamal Kant SahuGeorge Abraham
Dec 16, 2020·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Monique WilliamsPrue Talbot
Oct 18, 2019·Cancer Prevention Research·Boris MravecPavel Babal
Jan 12, 2021·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Dara Bracken-ClarkeStephen P Finn
Mar 23, 2021·Pharmacology & Therapeutics·Aleksandr B StefaniakStephen S Leonard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.