Epithelium and/or theca are required for ATP-elicited K+ current in follicle-enclosed Xenopus oocytes

Journal of Cellular Physiology
Carlos SaldañaR O Arellano

Abstract

The Xenopus follicular cell membrane is endowed with ATP-sensitive K+ channels, which are operated by various transmitters. These generate the ionic response named IK,cAMP via a mechanism that involves intracellular cAMP synthesis. It is known that opening these K+ channels favors oocyte maturation. Follicle stimulation by adenosine (Ado) or ATP consistently generates a strong IK,cAMP via activation of P1 and P3 purinergic receptors; however, ATP can also inhibit IK,cAMP, apparently acting on a third receptor type. Here, we show that IK,cAMP might be elicited by ATP released within the follicle, and that current activation by ATP was entirely dependent on the presence of epithelial and/or theca layers. Morphological studies confirmed that removal of epithelium/theca in these follicles (e.t.r.) was complete, and activation of fast Cl- (Fin) currents by ATP in e.t.r. follicles confirmed that communication between oocyte and follicular cells remained unchanged. Thus, dependence on epithelium/theca was specific for ATP-elicited K+ current. Using UTP and betagamma-MeATP as specific purinergic agents for IK,cAMP inhibition and activation, respectively, it was found that inhibition of IK,cAMP elicited by ATP or UTP was robustly presen...Continue Reading

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Citations

Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rogelio O ArellanoFrancisco Vázquez-Cuevas
Jul 16, 2010·Reproductive Biology and Endocrinology : RB&E·Francisco G Vázquez-CuevasRogelio O Arellano
Nov 26, 2013·Purinergic Signalling·Geoffrey Burnstock
May 20, 2015·Autonomic Neuroscience : Basic & Clinical·George I Gorodeski
Mar 17, 2006·Molecular Reproduction and Development·Francisco G Vázquez-CuevasRogelio O Arellano
Jan 22, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carlos SaldañaTeresa García-Gasca

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