Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane.

Journal of Agricultural and Food Chemistry
Nathan V MatusheskiElizabeth H Jeffery

Abstract

In some cruciferous plants, epithiospecifier protein (ESP) directs myrosinase (EC 3.2.3.1)-catalyzed hydrolysis of alkenyl glucosinolates toward epithionitrile formation. Here, for the first time, we show that ESP activity is negatively correlated with the extent of formation of the health-promoting phytochemical sulforaphane in broccoli (Brassica oleracea L. ssp. italica). A 43 kDa protein with ESP activity and sequence homology to the ESP of Arabidopsis thaliana was cloned from the broccoli cv. Packman and expressed in Escherichia coli. In a model system, the recombinant protein not only directed myrosinase-dependent metabolism of the alkenyl glucosinolate epi-progoitrin [(2S)-2-hydroxy-3-butenyl glucosinolate] toward formation of an epithionitrile but also directed myrosinase-dependent hydrolysis of the glucosinolate glucoraphanin [4-(methylsulfinyl)butyl glucosinolate] to form sulforaphane nitrile, in place of the isothiocyanate sulforaphane. The importance of this finding is that, whereas sulforaphane has been shown to have anticarcinogenic properties, sulforaphane nitrile has not. Genetic manipulation designed to attenuate or eliminate expression of ESP in broccoli could increase the fractional conversion of glucoraphanin...Continue Reading

References

Feb 28, 1997·Chemico-biological Interactions·D T VerhoevenG van Poppel
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J W FaheyP Talalay
Nov 24, 1999·Journal of Agricultural and Food Chemistry·M M KushadE H Jeffery
Feb 22, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R MithenJ Marquez

❮ Previous
Next ❯

Citations

Jun 17, 2008·European Journal of Nutrition·John D HayesIan M Eggleston
Jan 1, 2008·Journal of Agricultural and Food Chemistry·Subhendu MukherjeeDipak K Das
Apr 5, 2012·Journal of Agricultural and Food Chemistry·Grace C WangElizabeth H Jeffery
Jan 28, 2009·Journal of Agricultural and Food Chemistry·Jan F StevensStephen Machado
Feb 20, 2009·The Journal of Biological Chemistry·Ralph Kissen, Atle M Bones
Sep 25, 2008·Annual Review of Entomology·Richard J HopkinsJoop J A van Loon
Jul 31, 2012·BMC Evolutionary Biology·Jennifer C KuchernigUte Wittstock
Oct 25, 2008·PLoS Genetics·Adam M WentzellDaniel J Kliebenstein
Feb 3, 2011·World Journal of Cardiology·Sewon LeeCuihua Zhang
Oct 24, 2013·Nutrition Reviews·Christine A HoughtonJeff S Coombes
Feb 15, 2012·Meat Science·Rituparna BanerjeeH P Narkhede
Jul 28, 2009·Current Opinion in Chemical Biology·Aimee R Usera, Sarah E O'Connor
Dec 28, 2007·Journal of Biochemical and Biophysical Methods·Natalia BellostasHilmer Sørensen
Mar 11, 2008·Molecular Nutrition & Food Research·Elizabeth H Jeffery, Anna-Sigrid Keck
Aug 7, 2013·Journal of Food Science·Edward B Dosz, Elizabeth H Jeffery
Dec 18, 2013·Journal of the Science of Food and Agriculture·Kang Mo KuJohn A Juvik
Aug 4, 2009·Journal of Zhejiang University. Science. B·Gao-feng YuanQiao-mei Wang
Sep 13, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Klaus Peter LattéAlfonso Lampen
Mar 1, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Villatoro-PulidoM Del Río-Celestino
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shabir Ahmad Ganai
Dec 19, 2015·Journal of the Science of Food and Agriculture·Nan WangLianqing Shen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.