Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses

Archives of Virology
M OkumuraJiro Arikawa

Abstract

Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 mer YEDVNGIRK (NP 165-173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various hantavirus sequences bound MAb E5/G6 consistently, despite the existence of several amino acid variations in this region. These results indicate that MAb E5/G6 is a useful tool for detecting hantavirus antigen in rodent or patient tissues using Western blotting or other immunohistochemical assays.

Citations

Jun 14, 2008·Archives of Virology·Ichiro NakamuraJiro Arikawa
Aug 16, 2014·Viruses·Kumiko Yoshimatsu, Jiro Arikawa
Jan 5, 2010·The Journal of Veterinary Medical Science·Hiroaki KariwaIkuo Takashima
Dec 22, 2006·Clinical and Vaccine Immunology : CVI·Megumi OkumuraJiro Arikawa

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