PMID: 9422086Jan 9, 1998Paper

Epitope characterization of MUC1 antibodies

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
S ImaiY Kiyozuka

Abstract

The 56 MAbs submitted to the ISOBM TD-4 Workshop were analysed by ELISA assay for reactivity against synthetic MUC1 mucin protein core-related peptides. With peptides comprising 5, 7 and 20 amino acids, 4/56 (7.1%), 33/56 (58.9%) and 51/56 (91.1%) of antibodies showed positive reactivity, respectively. Peptides with 60, 80, 100 and 120 amino acids showed 50/56 (98.2%), 54/56 (96.4%), 53/56 (94.6%) and 47/56 (83.9%) antibodies with positive reactivity, respectively. The reactivity of each MAb with synthetic peptides was classified into 3 groups. In group A (15 MAbs), the reactivity increased depending on the peptides in length and was maximal with 20-, 60-, 80-, 100- and 120-mer peptides. In group B (24 MAbs), most antibodies showed very weak binding affinity to peptides with a small increase in reactivity with the 100- and 120-mer peptides. In group C (17 MAbs), the reactivity showed strong binding affinity with the 20-mer peptide followed by a reduced uneven pattern of reactivity with the 60-, 80-, 100- and 120-mer peptides. These results show that the binding affinity of MAbs against synthetic peptides does not depend on the recognition of antibodies to the epitopes of core protein.

Citations

Jun 22, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zach T BermanRebecca J Whelan

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