Epitope enhancement of a CD4 HIV epitope toward the development of the next generation HIV vaccine

The Journal of Immunology : Official Journal of the American Association of Immunologists
Takahiro OkazakiJ A Berzofsky

Abstract

Virus-specific CD4+ T cell help and CD8+ cytotoxic T cell responses are critical for maintenance of effective immunity in chronic viral infections. The importance of CD4+ T cells has been documented in HIV infection. To investigate whether a stronger CD4+ T cell response can be induced by modifications to enhance the T1 epitope, the first CD4+ T cell epitope discovered in HIV-1-gp120, we developed a T1-specific CD4+ T cell line from a healthy volunteer immunized with a canarypox vector expressing gp120 and boosted with recombinant gp120. This T1-specific CD4+ T cell line was restricted to DR13, which is common in U.S. Caucasians and African-Americans and very frequent in Africans. Peptides with certain amino acid substitutions in key positions induced enhanced specific CD4+ T cell proliferative responses at lower peptide concentration than the original epitope. This relatively conserved CD4 epitope improved by the epitope enhancement strategy could be a component of a more effective second generation vaccine construct for HIV infection.

References

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·R R PogueM Matsui
Sep 1, 1996·The Journal of Experimental Medicine·R D CardinP C Doherty
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J D AhlersJ A Berzofsky
Dec 22, 1998·The Journal of Experimental Medicine·A J ZajacR Ahmed
Oct 12, 2000·Nature·E S RosenbergB D Walker
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A C McNeilM Connors
Mar 22, 2002·Nature Reviews. Immunology·J A BerzofskyI M Belyakov
Jan 8, 2003·The Journal of Gene Medicine·Matilu Mwau, Andrew J McMichael
Jan 14, 2003·Annual Review of Immunology·Daniel C DouekRichard A Koup
Feb 21, 2003·Nature·Edith M JanssenStephen P Schoenberger
Apr 12, 2003·Science·Susan M Kaech, Rafi Ahmed
Apr 12, 2003·Science·Joseph C Sun, Michael J Bevan
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takahiro OkazakiJay A Berzofsky

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