Abstract
A panel of 56 murine monoclonal antibodies against the MUC1 mucin was analysed for reactivity against purified MUC1 mucin and synthetic MUC1 mucin protein core-related peptides. In an ELISA assay, with purified normal urinary mucin as the target antigen, 48/56 (86%) of antibodies showed positive reactivity. A smaller proportion of antibodies, 31/56 (55%), reacted with a bovine serum albumin conjugate containing the synthetic peptide, APDTRPAPG. This peptide represents the hydrophilic region of the tandem repeat sequence of the MUC1 mucin core. These 31 anti-mucin core antibodies were then evaluated for reactivity with a set of overlapping heptamers with sequences based upon that of the 20-amino acid tandem repeat of the MUC1 protein core. For this purpose, the 'Pepscan' procedure was employed to detect antibody binding to peptides tethered at their carboxy termini to the polypropylene pins. Synthetic peptides binding to these antibodies were identified for 29 of the samples provided and epitopes or 'minimum binding units' were deduced for these. Most antibodies bound to epitopes of 4 amino acid residues with the sequence RPAP occurring most frequently. The results confirm that the short hydrophilic region PDTRPAP in the MUC1 mu...Continue Reading
Citations
Jul 11, 2003·Immunology Letters·Paul N NelsonFrank C Hay
Mar 5, 2002·European Journal of Biochemistry·Heiko MöllerUNKNOWN Bernd Meyer
Feb 13, 2002·Hybridoma and Hybridomics·W QiR Madiyalakan
Oct 4, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Martin KirchmairSigrid Neuhauser
Aug 25, 2001·Analytical Biochemistry·A MurrayM R Price
Jul 26, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Alice L SilvaRicardo F Savaris
Mar 17, 2001·Cancer·E BriasoulisN Pavlidis
Apr 22, 2004·Journal of Biotechnology·T LinkT Noll
May 20, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·María V CroceAmada Segal-Eiras
Jul 24, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan E BaldusFranz-Georg Hanisch
Nov 3, 2005·British Journal of Cancer·L M PericleousM P Deonarain
Oct 9, 2015·Applied Immunohistochemistry & Molecular Morphology : AIMM·Ledamir R BritoRicardo F Savaris
May 20, 2020·Frontiers in Immunology·Velizar ShivarovAnastas D Pashov
May 8, 2000·International Journal of Cancer. Journal International Du Cancer·S von Mensdorff-PouillyJ Hilgers
Mar 14, 2002·BJU International·M S SimmsM C Bishop
Jan 17, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·A Marina Hinojosa-KurtzbergSandra J Gendler
Apr 22, 2020·Cancer Chemotherapy and Pharmacology·Geraldine O 'Sullivan CoyneJames H Doroshow
Feb 20, 2021·Analytical Methods : Advancing Methods and Applications·Shane Patrick FlanaganJanice Limson