PMID: 16637277Apr 28, 2006Paper

Epitope mapping of antigenic determinants of hepatitis C virus proteins by phage display

Molekuliarnaia biologiia
E A RechkinaA A Kushch

Abstract

Study of individual hepatitis C (HCV) proteins could help to find a molecular structure and conformation, localization of antigenic and immunogenic determinants, to reveal of protective epitopes. It is necessary for practical medicine - development of diagnostic test-systems, vaccines and therapeutics. Linear and conformation dependent epitopes of HCV proteins was localized in this work and immunogenic properties of phage displayed peptides screened on monoclonal antibodies to HCV proteins have been investigated. Eleven epitopes of four HCV proteins have been studied. Three epitopes was found as linear, two epitopes were dependent on secondary structure of proteins and one epitope was dependent on tertiary structure of NS3 protein. Aminoacid sequences of other determinants have been determined and the distinct localization of these determinants will be continued after discovering of tertiary structure of HCV proteins. It was shown, that phage mimotope 3f4 is immunogenic and could induce specific hu- moral immune response to NS5A HCV protein. The data obtained could be useful for improving of HCV diagnostic test-systems, studying of amino acid substitutions and its influence on antigenic properties of the HCV proteins. The resul...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W M ChingJ W Shih
Jul 27, 1990·Science·J K Scott, G P Smith
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D MoradpourH E Blum
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M ChenD R Milich
Jan 5, 2002·Journal of Clinical Microbiology·Xiao-Guang DouYury E Khudyakov
Nov 5, 2003·Journal of Molecular Biology·David Enshell-SeijffersJonathan M Gershoni
Jan 30, 2004·Journal of Medical Virology·Colette Jolivet-ReynaudDominique Rolland

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Citations

Nov 9, 2017·International Journal of Molecular Sciences·Olga V MasalovaAlla A Kushch

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