PMID: 9637294Jun 24, 1998Paper

Epitope mapping of bovine leukemia virus transactivator protein Tax

The Journal of Veterinary Medical Science
N SakakibaraM Onuma

Abstract

The immunogenicity of the bovine leukemia virus (BLV) transactivator protein (tax) was studied by mapping its B-cell and T-cell epitopes. Peptides (18 to 20-mer) overlapping by 10 amino acids, spanning whole amino acid sequence of BLVtax were synthesized. Recombinant BLVtax protein was used to immunize two different strains of mice, C57BL/6 and BALB/c. B-cell and T-cell epitopes of recombinant BLVtax protein was determined by screening all the 30 synthetic peptides, against immune serum in ELISA for antibody reactivity, and against immune spleen cells in lymphocyte proliferation assay for T-cell stimulation. Peptides with amino acids at position 111-130 and 131-150 were T-cell epitopes for C57BL/6 and BALB/c mice immune cells, respectively. B-cell epitope was mapped to amino acid sequence at 261-280 in both strains of mice. These results imply that BLVtax protein contains some of BLV- immunodominant epitopes and this information may be applied for designing an effective peptide vaccine capable of inducing neutralizing antibodies as well as cellular immunity.

References

Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N SagataY Ikawa
Dec 1, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M C ReM La Placa
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·P HöllsbergD A Hafler
Dec 1, 1995·Immunology Today·S HaraguchiN K Day

❮ Previous
Next ❯

Citations

Apr 7, 2007·Autoimmunity Reviews·Darja KanducAbraham Mittelman
Dec 11, 2003·The Journal of Veterinary Medical Science·Tatsufumi UsuiMisao Onuma
May 30, 2006·Virus Genes·Kathleen Margaret McGirr, Gertrude Case Buehuring
Feb 11, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·K M McGirr, G C Buehring

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.