Epitope Mapping of Streptococcus agalactiae Elongation Factor Tu Protein Recognized by Human Sera

Frontiers in Microbiology
Marcelina PyclikMonika Brzychczy-Włoch

Abstract

The elongation factor Tu has been identified as one of the most immunoreactive proteins that was recognized by human sera of GBS (group B streptococcus) positive patients. In this paper, we present the polypeptide-specific epitopes of the bacterial protein that are recognized by human antibodies: 28LTAAITTVLARRLP41 (peptide no. 3) and 294GQVLAKPGSINPHTKF309 (peptide no. 21). To determine the shortest amino acid sequence recognized by antibodies, truncation peptide libraries were prepared using the PEPSCAN method. The analysis of immunoreactivity of peptides with sera of GBS positive and negative women revealed that the most immunoreactive sequence was 306HTKF309. Moreover, we observed that this sequence also showed the highest specificity which was based on ratio of reactivity with sera of GBS positive relative to sera of GBS negative patients. Epitope was synthetized on Wang resin with the Fmoc strategy. Our results open the possibility to use 306HTKF309 peptide in diagnostic assays to determine Streptococcus agalactiae infection in humans.

References

Dec 10, 1990·FEBS Letters·A S Kolaskar, P C Tongaonkar
Sep 24, 1987·Journal of Immunological Methods·H M GeysenP G Schoofs
Aug 1, 1986·Journal of Clinical Microbiology·H B RatnerC W Stratton
Apr 18, 2001·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M VotavaI Braveny
Nov 8, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C GuerreroM Segovia
Apr 26, 2006·Immunome Research·Jens Erik Pontoppidan LarsenMorten Nielsen
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Pernille Haste AndersenOle Lund
May 19, 2007·Diagnostic Microbiology and Infectious Disease·Jennifer S Goodrich, Melissa B Miller
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anja KunertPeter F Zipfel
Feb 7, 2013·The Pediatric Infectious Disease Journal·Alberto Berardi Emilia-Romagna
Aug 30, 2013·Vaccine·Atul Kumar JohriSteven Black
Jul 8, 2015·Frontiers in Genetics·Constance J Jeffery
Jan 1, 2015·Emerging Microbes & Infections·Sebastian E CarrascoX Frank Yang
Sep 13, 2017·Scientific Reports·Michael WidjajaSteven Philip Djordjevic

❮ Previous
Next ❯

Methods Mentioned

BETA
immunosorbent assay
enzyme-linked immunosorbent assay
ELISA
PCR
electrophoresis

Software Mentioned

GraphPad
Emini
Genesilico Metaserver
Prism
PEPSCAN
Bepipred
Mascot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved