Epitope mapping of the monoclonal antibody FAC2 on the apoprotein of Cpa-1 in photosystem II.

FEBS Letters
L K Frankel, T M Bricker

Abstract

Using a combination of cyanogen bromide cleavage and endoproteinase digestion we have shown that the putative epitope for the monoclonal antibody FAC2 lies in the region 360Pro(-391)Ser on the apoprotein of CPa-1. This region lies entirely within the large extrinsic loop of this protein. We have shown previously that the epitope of FAC2 becomes exposed in oxygen-evolving membranes upon treatment with alkaline Tris which releases all four of the manganese associated with the oxygen-evolving site of photosystem II. The epitope is not exposed, however, after CaCl(2) treatment and exposure to low concentrations of chloride, conditions which lead to the release of two of the four manganeses associated with the oxygen-evolving site. These results suggest that, upon release of the chloride-insensitive manganese from photosystem II membranes, a conformational change occurs which leads to the exposure of 360Pro(-391)Ser on CPa-1 to the monoclonal antibody FAC2.

References

Sep 1, 1979·Analytical Biochemistry·V Nikodem, J R Fresco
Jul 1, 1987·Archives of Biochemistry and Biophysics·T M Bricker, L K Frankel
Apr 17, 1989·Biochimica Et Biophysica Acta·D F GhanotakisC F Yocum
Jan 30, 1986·Biochimica Et Biophysica Acta·J K Mohana Rao, P Argos
Jul 1, 1988·European Journal of Biochemistry·G von Heijne, Y Gavel
Feb 15, 1985·Archives of Biochemistry and Biophysics·T M BrickerL A Sherman
Sep 1, 1983·Infection and Immunity·K S Wise, R K Watson
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Delepelaire, N H Chua
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O Nanba, K Satoh

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Citations

Jul 25, 2012·Plant Physiology and Biochemistry : PPB·Mayank Anand GururaniSe Won Park
May 21, 2016·Frontiers in Plant Science·Han Bao, Robert L Burnap
Feb 15, 2000·The Journal of Biological Chemistry·L B AndersonB A Barry
Oct 16, 1992·Biochimica Et Biophysica Acta·R J Debus

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