Epitope of OSCP oligomycin sensitivity conferring protein exposed at the surface of the mitochondrial ATPase-ATPsynthase complex.

Biochimie
C GodinotB Roux

Abstract

Immunological studies were designed to study the structure of the oligomycin sensitivity conferring protein (OSCP) integrated in the mitochondrial ATPase-ATPsynthase complex. The monoclonal antibody 2B1B1 used in this study could bind as well to purified or membrane bound OSCP as shown previously by Protein A-gold immunocytochemistry and by competitive immunotitration. In this paper, it is shown that 2B1B1 can also immunoprecipitate the F0F1 complex from a Triton X-100 extract. This means that not only, 2B1B1 binds to the surface of OSCP but also that the binding of 2B1B1 did not destroy the interactions between F0 and F1 and further demonstrates the external location of the 2B1B1 binding site in the ATPase-ATPsynthase complex. This antigenic site was located on the N-terminal sequence of OSCP, between residues 1 and 72, as demonstrated after chemical cleavage of OSCP with formic acid, hydroxylamine and partial cleavage with cyanogen bromide. The proximity of Tyr and Arg to the epitope was suggested by the lack of 2B1B1 binding to iodinated OSCP and by the susceptibility of this binding to trypsin or to endoproteinase Arg-C treatments of OSCP, respectively. A more precise location of the epitope has been attempted by using the ...Continue Reading

References

Feb 1, 1977·Journal of Clinical Pathology·W K Blenkinsopp, G P Haffenden
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Nov 1, 1985·Molecular Immunology·E A Padlan
Aug 1, 1988·Computer Applications in the Biosciences : CABIOS·G DeléageD C Gautheron
Aug 29, 1986·Biochemical and Biophysical Research Communications·F Vonderviszt, I Simon
Jan 1, 1983·Annual Review of Biochemistry·L M Amzel, P L Pedersen
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Apr 1, 1994·Physical Review. B, Condensed Matter·P MendelsC E Stronach

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.