Epitope recognition by anti-cathepsin D autoantibodies in endometrial cancer patients

Gynecologic Oncology
J R BosscherD D Taylor

Abstract

This study analyzed a model for the identification of specific epitopes recognized by autologous tumor-reactive humoral responses of endometrial cancer patients as potential markers for the monitoring of cancer. The presence of circulating pro- and mature forms of cathepsin D and antibodies reactive with this enzyme were identified by Western immunoblot and quantitated by an enzyme immunoassay. Specific immunoreactivities with 34- and 52-kDa cathepsin D forms were analyzed by Western immunoblot using sera from endometrial cancer patients (n = 40) and normal volunteers (n = 15). Subsequently, reactivities with specific cathepsin D epitopes were defined by a peptide-specific ELISA. Circulating pro-forms of cathepsin D were detected in 31 of 40 endometrial cancer patients tested and none of the control volunteers. Circulating IgG reactive with cathepsin D could be demonstrated in 29/31 patients with circulating procathepsin D, while an anti-cathepsin D response was not detectable in normal controls. This response appeared to be directed against the pro-peptide portion of cathepsin D. Using a peptide-specific ELISA, the frequencies of antibody production against specific epitopes within the pro-peptide were defined. There is a demo...Continue Reading

References

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Citations

Feb 1, 2011·Current Opinion in Immunology·Daniel W Cramer, Olivera J Finn
Aug 4, 2009·Gynecologic Oncology·Douglas D TaylorLynn P Parker
Jun 11, 2009·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Nicole Brooks, Dodie S Pouniotis
May 9, 2009·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jae Kyo YiDong-Young Noh
May 16, 2021·Journal of Hematology & Oncology·Ziqi ZhangXiawei Wei

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