PMID: 6425829Apr 1, 1984Paper

Epitope-specific protective immunogenicity of chemically synthesized 13-, 18-, and 23-residue peptide fragments of streptococcal M protein

Proceedings of the National Academy of Sciences of the United States of America
E H BeacheyL Chedid

Abstract

The ability of chemically synthesized subpeptides of type 24 streptococcal M protein to evoke protective antibodies in rabbits was investigated. We synthesized copies of the COOH-terminal 13, 18, and 23 amino acid residues of cyanogen bromide fragment 7 (CB7) of pepsin-extracted type 24 M protein, except that methionine was substituted for homoserine as the COOH-terminal residue. An additional residue of cysteine was added at the COOH terminus of the 13-residue peptide. Each of the peptides, designated S-CB7-(23-35)-Cys, S-CB7-(18-35), and S-CB7-(13-35), when conjugated to lysylated tetanus toxoid with glutaraldehyde, was capable of stimulating formation of protective anti-type 24 M protein antibodies in rabbits. The smallest peptide, S-CB7-(23-35)-Cys, elicited immune responses equally as strong, if not stronger, than those to the longer peptides. A single Lys/Gly substitution in this 13-residue peptide resulted in its failure to stimulate protective antibodies. None of the antisera reacted with heterologous serotypes of M protein and none reacted with frozen sections of human heart tissue. These results indicate that a chemically synthesized peptide fragment corresponding to as few as 13 amino acid residues of streptococcal M...Continue Reading

References

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Citations

Sep 3, 1990·Biochimica Et Biophysica Acta·M KotbE H Beachey
Apr 1, 1988·Vaccine·E H BeacheyS Sargent
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W Kraus, E H Beachey
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P AskelöfT Bartfai
Jan 1, 1985·The Journal of Experimental Medicine·J B Dale, E H Beachey
Mar 1, 1985·The Journal of Experimental Medicine·K F JonesV A Fischetti
May 1, 1986·The Journal of Experimental Medicine·J B Dale, E H Beachey
Feb 1, 1989·The Journal of Experimental Medicine·W KrausE H Beachey
Mar 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·O KühnemundW Köhler
Mar 1, 1992·Microbial Pathogenesis·H S CourtneyD L Hasty
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti
Dec 1, 1992·Infection and Immunity·P P ClearyJ Handley
Aug 1, 1989·Infection and Immunity·W KrausE H Beachey
Nov 1, 1991·Clinical Immunology and Immunopathology·G H Stollerman

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