Epitope specificity and isoforms of the mycobacterial 19-kilodalton antigen.

Infection and Immunity
D HarrisJ Ivanyi

Abstract

The topography and specificity of B- and T-cell stimulatory epitopes from the 19-kDa protein of Mycobacterium tuberculosis were investigated by using overlapping synthetic peptides. Murine antisera identified two cryptic epitopes (residues 11 to 30 and 61 to 80) and one species-specific immunodominant epitope (residues 140 to 159). Immunoglobulins G1 and G2a antibody isotypes varied for the respective peptide immunogens but without relationship to the T-cell cytokine profiles which were characterized by high gamma interferon and low interleukin 5 levels. Antisera to recombinant M. tuberculosis 19-kDa protein (rGST-19) cross-reacted with homologous proteins of similar size from organisms of the Mycobacterium avium-intracellulare complex. Two-dimensional gel electrophoresis revealed differences in the number, relative mobility, and charge of isoforms of the 19-kDa protein, possibly reflecting posttranslational modifications. The immunodominant T-cell epitope from the M. tuberculosis 19-kDa protein (residues 61 to 80) and the corresponding peptide sequence from Mycobacterium avium subsp. intracellulare (residues 64 to 83), differing at five residues, were both recognized in a genetically permissive manner. Peptides 61-80 and 64-83...Continue Reading

References

Oct 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G BothamleyJ Ivanyi
Sep 1, 1991·Infection and Immunity·D B Young, T R Garbe
Jan 1, 1991·Research in Microbiology·D B Young, T R Garbe
Jul 1, 1990·Journal of General Microbiology·M E CollinsJ W Dale
Dec 1, 1988·European Journal of Immunology·J H CoxM J Francis
Nov 1, 1988·Journal of Clinical Microbiology·P S JackettJ Ivanyi
Feb 11, 1989·Nucleic Acids Research·K R AshbridgeR B Lathigra
Jan 17, 1989·Journal of Immunological Methods·T R Mosmann, T A Fong

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