PMID: 7520897Apr 1, 1994Paper

Epitope specificity of HLA class I alloantibodies. I. Frequency analysis of antibodies to private versus public specificities in potential transplant recipients

Human Immunology
G E RodeyR A Bray

Abstract

Sera obtained sequentially from 419 patients awaiting solid organ transplantation were screened and analyzed for HLA class I epitope specificity. Antibodies detected in each serum were defined as "private" if reactivity could only be demonstrated against a single specificity within one of the eight major CREGs, or as "public" if reactivity in a serum could be demonstrated against two or more specificities within a single CREG. A total of 139 sera contained % PRA > 0, in which 147 specific antibodies were identified. Of the 103 positive sera, 93 (90%) contained antipublic antibodies, with or without additional antiprivate antibodies, whereas just 10 (10%) sera contained only apparent antiprivate antibodies. The success rate in defining antibody specificities was low at PRA values of 1%-20% due to weak reactivity and high false-positive rates. Specificity analysis with high test sensitivity and specificity was achieved with PRA values between 40% and 80%. At PRA values > 80%, test sensitivity remained high but specificity declined. We conclude that most anti-HLA antibodies are directed against high frequency public epitope clusters (CREGs), and highly sensitized patients develop antibodies in a fairly predictable fashion, a featu...Continue Reading

References

Nov 1, 1976·European Journal of Immunology·J Ayres, P Cresswell
Oct 1, 1979·The Journal of Clinical Investigation·B D SchwartzG E Rodey
May 1, 1992·Human Immunology·J G BodmerT Sasazuki
Jul 1, 1990·Human Immunology·A A FullerT C Fuller
Feb 1, 1986·Transplantation·Y KonoedaM R Mickey
Oct 15, 1989·Cellular Immunology·C López-Larrea, A Arnaiz-Villena
Sep 15, 1988·Nature·F FigueroaJ Klein

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Citations

Mar 17, 2000·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·R G SawyerR B Isaacs
May 22, 1998·Transplantation Proceedings·A C PapassavasC Stavropoulos-Giokas
Mar 20, 1999·Transplantation Proceedings·A C PapassavasC Stavropoulos-Giokas
Aug 1, 1997·Human Immunology·N SteinerC K Hurley
Aug 1, 1997·Human Immunology·J M Cecka
Feb 23, 1999·Human Immunology·E HsuC K Hurley
Oct 26, 2000·Clinical Transplantation·P C RodríguezL F García
Oct 3, 2002·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·A C PapassavasA Kostakis
Nov 26, 1999·Kidney International·Y W SijpkensL C Paul
Aug 10, 2000·Transplantation·A C PapassavasC Stavropoulos-Giokas
Aug 19, 2000·Transplantation·C S HollenbeakM A Schnitzler
Sep 28, 2007·Current Opinion in Hematology·Ralph R Vassallo
Sep 10, 2002·Transplant Immunology·Frans H J Claas
Feb 16, 2016·Transfusion Medicine·W XiaS Santoso
Feb 13, 2016·Frontiers in Immunology·Michelle J HickeyElaine F Reed
Sep 21, 2010·Transfusion Medicine Reviews·Elianna SaidenbergDonald R Branch
Mar 13, 2016·Revista brasileira de hematologia e hemoterapia·Carolina Bonet BubJosé Mauro Kutner
Sep 4, 2004·Clinical Transplantation·Inmaculada DíazFrancisco Valdés
Oct 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J CaiL Rebellato

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