Epitope specificity of Th0/Th2 CD4+ T-lymphocyte clones induced by vaccination with rHBsAg vaccine

Gastroenterology
M C HonoratiA Facchini

Abstract

Different amino acid sequences of hepatitis B virus surface antigen (HBsAg) are involved in the activation of CD4+ lymphocytes needed to induce an optimal antiviral function. The aim of this study was to characterize the CD4-mediated response to immunodominant HBsAg epitopes in hepatitis B virus (HBV) vaccine recipients by defining minimal sequences recognized by T cells, cytokine profiles, and HLA restriction of peptide recognition. T-lymphocyte lines and clones specific for HBsAg were isolated from the peripheral blood of subjects immunized with recombinant HBsAg and stimulated in vitro with synthetic peptides spanning the whole HBsAg sequence. Four immunodominant epitopes (sequences 21-40, 136-155, 156-175, and 211-226) were identified. Using panels of truncated peptides of different length, sequences 21-28, 165-172, and 215-223 were shown to correspond to the minimal epitopes recognized by T cells. The antigen-specific T-lymphocyte proliferation was HLA class II restricted, and each peptide could be presented in association with different HLA class II determinants. Th0/Th2 cytokine patterns were induced on peptide stimulation. These results indicate the presence of at least four immunodominant epitopes within HBsAg that rep...Continue Reading

Citations

May 1, 2004·AIDS Research and Human Retroviruses·Philip J NorrisEric S Rosenberg
Jun 21, 2008·World Journal of Gastroenterology : WJG·Vijayakumar VeluSadras-Panchatcharam Thyagarajan
Nov 11, 2003·Clinical and Diagnostic Laboratory Immunology·Patricia AguilarAnne Kennel De March
May 26, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andrew GodkinAdrian V S Hill
Apr 6, 2005·Clinical and Experimental Immunology·A KrugerT Hoehler
Feb 3, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F RahmanH F Löhr
Jan 31, 2002·World Journal of Gastroenterology : WJG·Maria Cristina Honorati, Andrea Facchini
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tanja BauerWolfgang Jilg
May 11, 2021·Clinical and Experimental Immunology·Tamsin Cargill, Eleanor Barnes

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