Epitope topography controls bioactivity in supramolecular nanofibers

Biomaterials Science
Shantanu SurSamuel I Stupp

Abstract

Incorporating bioactivity into artificial scaffolds using peptide epitopes present in the extracellular matrix (ECM) is a well-known approach. A common strategy has involved epitopes that provide cells with attachment points and external cues through interaction with integrin receptors. Although a variety of bioactive sequences have been identified so far, less is known about their optimal display in a scaffold. We report here on the use of self-assembled peptide amphiphile (PA) nanofiber matrices to investigate the impact of spatial presentation of the fibronectin derived epitope RGDS on cell response. Using one, three, or five glycine residues, RGDS epitopes were systematically spaced out from the surface of the rigid nanofibers. We found that cell morphology was strongly affected by the separation of the epitope from the nanofiber surface, with the longest distance yielding the most cell-spreading, bundling of actin filaments, and a round-to-polygonal transformation of cell shape. Cell response to this type of epitope display was also accompanied with activated integrin-mediated signaling and formation of stronger adhesions between cells and substrate. Interestingly, unlike length, changing the molecular flexibility of the l...Continue Reading

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Citations

Feb 9, 2016·Soft Matter·S MotamedM I Aguilar
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Dec 20, 2019·Biomaterials Science·Amber M HilderbrandApril M Kloxin
Jan 16, 2021·Advanced Drug Delivery Reviews·Matthew J Webber, E Thomas Pashuck

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