PMID: 9524192May 9, 1998Paper

Epitopes of components of the plasminogen activation system are re-exposed in formalin-fixed paraffin sections by different retrieval techniques

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
C M FerrierG N van Muijen

Abstract

We present a systematic analysis of the sensitivity and specificity of immunohistochemical stainings for components of the plasminogen activation system, i.e., uPA, tPA, PAI-1, PAI-2, and uPAR, on routinely processed (formalin-fixed, paraffin-embedded) tissues. Five to nine antibodies per component were tested and the influence of different antigen retrieval regimens on immunoreactivity was investigated. We studied six different microwave-mediated pretreatments and two pretreatments by proteolytic digestion. First, positive and negative control tissues were stained. Then, frozen and paraffin sections from the same cancer lesions were stained after specific modes of pretreatment and with selected antibodies. For each component, one or a few of the tested Abs gave optimal staining on paraffin sections when combined with a particular tissue pretreatment. For PAI-1, and to a lesser degree also for tPA, an underrepresentation of stromal cell staining in paraffin material was found, whereas tumor cells showed good staining. For uPA, PAI-2, and uPAR, consistent staining results were obtained on paraffin sections.

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Citations

Sep 5, 2002·The Journal of Pathology·Cilia M FerrierDirk J Ruiter
Dec 16, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D J RuiterM Schmitt
Dec 31, 2003·Methods : a Companion to Methods in Enzymology·J Alain KummerC Erik Hack
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·G Mayer, M Bendayan
Jan 2, 2007·Progress in Histochemistry and Cytochemistry·Shuji Yamashita
Oct 28, 2003·The American Journal of Pathology·Birgitte Vrou OffersenPeter A Andreasen
Jul 25, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J A Ramos-Vara, M E Beissenherz
Jul 13, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Katsura EmotoYasunori Okada
Jun 9, 2001·Applied Immunohistochemistry & Molecular Morphology : AIMM·S R ShiC R Taylor
Jun 20, 2002·Orthodontics & Craniofacial Research·P ZeitlerH Collmann
Oct 10, 2003·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Z KahveciI Cavusoglu

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