Epitranscriptomic 5-Methylcytosine Profile in PM2.5 -induced Mouse Pulmonary Fibrosis

Genomics, Proteomics & Bioinformatics
Xiao HanWenjun Ding

Abstract

Exposure of airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM2.5) is epidemiologically associated with lung dysfunction and respiratory symptoms, including pulmonary fibrosis. However, whether epigenetic mechanisms are involved in PM2.5-induced pulmonary fibrosis is currently poorly understood. Herein, using a PM2.5-induced pulmonary fibrosis mouse model, we found that PM2.5 exposure leads to aberrant mRNA 5-methylcytosine (m5C) gain and loss in fibrotic lung tissues. Moreover, we showed the m5C-mediated regulatory map of gene functions in pulmonary fibrosis after PM2.5 exposure. Several genes act as m5C gain-upregulated factors, probably critical for the development of PM2.5-induced fibrosis in mouse lungs. These genes, including Lcn2, Mmp9, Chi3l1, Adipoq, Atp5j2, Atp5l, Atpif1, Ndufb6, Fgr, Slc11a1, and Tyrobp, are highly related to oxidative stress response, inflammatory responses, and immune system processes. Our study illustrates the first epitranscriptomic RNA m5C profile in PM2.5-induced pulmonary fibrosis and will be valuable in identifying biomarkers for PM2.5 exposure-related lung pathogenesis with translational potential.

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Citations

Sep 29, 2020·Frontiers in Cell and Developmental Biology·Gangqiang GuoXiangyang Xue
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Gangqiang GuoXiangyang Xue

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Datasets Mentioned

BETA
GSE122493
PRJCA001116

Methods Mentioned

BETA
RNA-Seq
electrophoresis

Software Mentioned

BisSeq
IGVTools
IGV
meRanT align
Trimmomatic
DEGseq
DAVID
Integrative Genomics viewer
intersectBed
RNA

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