Epizootic hemorrhagic disease virus serotype 7 in European cattle and sheep: diagnostic considerations and effect of previous BTV exposure

Veterinary Microbiology
Michael EschbaumerBernd Hoffmann

Abstract

Epizootic hemorrhagic disease virus (EHDV), an arthropod-borne orbivirus (family Reoviridae), is an emerging pathogen of wild and domestic ruminants that is closely related to bluetongue virus (BTV). The present study examines the outcome of an experimental EHDV-7 infection of Holstein cattle and East Frisian sheep. Apart from naïve animals that had not been exposed to BTV, it included animals that had been experimentally infected with either BTV-6 or BTV-8 two months earlier. In addition, EHDV-infected cattle were subsequently challenged with BTV-8. Samples were tested with commercially available ELISA and real-time RT-PCR kits and a custom NS3-specific real-time RT-PCR assay. Virus isolation was attempted in Vero, C6/36 and KC cells (from Culicoides variipennis), embryonated chicken eggs and type I interferon receptor-deficient IFNAR(-/-) mice. EHDV-7 productively infected Holstein cattle, but caused no clinical signs. The inoculation of East Frisian sheep, on the other hand, apparently did not lead to a productive infection. The commercial diagnostic kits performed adequately. KC cells proved to be the most sensitive means of virus isolation, but viremia was shorter than 2 weeks in most animals. No interference between EHDV ...Continue Reading

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Sep 17, 2011·Veterinary Microbiology·Michael EschbaumerBernd Hoffmann

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Citations

Aug 1, 2013·Research in Veterinary Science·Emmanuel BreardStéphan Zientara
Feb 24, 2015·The Journal of General Virology·William C WilsonElizabeth Howerth
Jun 19, 2015·Vector Borne and Zoonotic Diseases·Barbara S DroletPeter P Mertens
Jul 31, 2016·Research in Veterinary Science·Mario ForzanFrancesco Tolari
Jun 4, 2020·Microorganisms·Lesley Bell-SakyiPeter P C Mertens
Sep 1, 2021·Vector Borne and Zoonotic Diseases·Katsunori MurotaTohru Yanase

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