PMID: 8972632Dec 1, 1996Paper

EPR detection of free radicals in UV-irradiated skin: mouse versus human

Photochemistry and Photobiology
B A Jurkiewicz, G R Buettner

Abstract

Ultraviolet radiation produces free radicals in Skh-1 mouse skin, contributing to photoaging and carcinogenesis. If a mouse model is a general indicator of free radical processes in human skin photobiology, then radical production observed in mouse and human skin should be directly comparative. In this work we show that UV radiation (lambda > 300 nm, 14 microW/cm2 UVB; 3.5 mW/cm2 UVA) increases the ascorbate free radical (Asc.-) electron paramagnetic resonance (EPR) signal in both Skh-1 mouse skin (45%) and human facial skin biopsies (340%). Visible light (lambda > 400 nm; 0.23 mW/cm2 UVA) also increased the Asc.- signal in human skin samples (45%) but did not increase baseline mouse Asc.-, indicating that human skin is more susceptible to free radical formation and that a chromophore for visible light may be present. Using EPR spin-trapping techniques, UV radiation produced spin adducts consistent with trapping lipid alkyl radicals in mouse skin (alpha-[4-pyridyl 1-oxide]-N-tert-butyl nitrone/alkyl radical adduct; aN = 15.56 G and aH = 2.70 G) and lipid alkoxyl radicals in human skin (5,5-dimethylpyrroline-1-oxide/alkoxyl radical adduct; aN = 14.54 G and aH = 16.0 G). Topical application of the iron chelator Desferal to human ...Continue Reading

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Citations

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