EPSPS Gene Amplification in Glyphosate-Resistant Italian Ryegrass (Lolium perenne ssp. multiflorum) Populations from Arkansas (United States)

Journal of Agricultural and Food Chemistry
Reiofeli A SalasNilda R Burgos

Abstract

Glyphosate-resistant Italian ryegrass was detected in Arkansas (United States) in 2007. In 2014, 45 populations were confirmed resistant in eight counties across the state. The level of resistance and resistance mechanisms in six populations were studied to assess the severity of the problem and identify alternative management approaches. Dose-response bioassays, glyphosate absorption and translocation experiments, herbicide target (EPSPS) gene sequence analysis, and gene amplification assays were conducted. The dose causing 50% growth reduction (GR50) was 7-19 times higher for the resistant population than for the susceptible standard. Uptake and translocation of (14)C-glyphosate were similar in resistant and susceptible plants, and no mutation in the EPSPS gene known to be associated with resistance to glyphosate was detected. Resistant plants contained from 11- to >100-fold more copies of the EPSPS gene than the susceptible plants, whereas the susceptible plants had only one copy of EPSPS. Plants surviving the recommended dose of glyphosate contained at least 10 copies. The EPSPS copy number was positively related to glyphosate resistance level (r = 80). Therefore, resistance to glyphosate in these populations is due to mult...Continue Reading

References

Jan 1, 1986·Archives of Biochemistry and Biophysics·H C SteinrückenR T Fraley
Jun 30, 1980·Biochemical and Biophysical Research Communications·H C Steinrücken, N Amrhein
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·E SchönbrunnW Kabsch
Jul 28, 2001·Physiologia Plantarum·Jack M. WidholmJeffrey E. Brotherton
Feb 27, 2007·Planta·Alejandro Perez-JonesCarol A Mallory-Smith
Feb 15, 2008·Pest Management Science·Stephen B Powles
Feb 15, 2008·Pest Management Science·Stephen O Duke, Stephen B Powles
Sep 27, 2008·Pakistan Journal of Biological Sciences : PJBS·J L ChongS Salmijah
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Todd A GainesPhilip Westra
Jan 12, 2010·Pest Management Science·Xia GeR Douglas Sammons
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephen B Powles
Mar 3, 2010·Annual Review of Plant Biology·Stephen B Powles, Qin Yu
Feb 19, 2011·Journal of Agricultural and Food Chemistry·Todd A GainesPhilip Westra
Dec 20, 2011·Journal of Agricultural and Food Chemistry·Leonardo Bianco de CarvalhoMaría Dolores Luque de Castro
Mar 14, 2012·Nature Reviews. Genetics·Christine Vogel, Edward M Marcotte
Sep 15, 2012·Proceedings. Biological Sciences·Fyodor A Kondrashov

❮ Previous
Next ❯

Citations

Dec 15, 2015·Journal of Agricultural and Food Chemistry·Manuel Fernández-EscaladaMercedes Royuela
Aug 30, 2016·Frontiers in Plant Science·Pablo T Fernández-MorenoRafael De Prado
Oct 16, 2016·The Plant Journal : for Cell and Molecular Biology·Jingchao ChenChaoxian Zhang
Mar 8, 2017·Pest Management Science·Christopher R Van HornTodd A Gaines
Dec 1, 2017·Frontiers in Plant Science·Enzo R BracamonteRafael De Prado
Dec 7, 2018·Pest Management Science·Caio Augusto De Castro Grossi BrunharoBradley D Hanson
May 19, 2020·Pest Management Science·Vijay SinghMuthukumar Bagavathiannan
Nov 2, 2017·Frontiers in Plant Science·Pablo T Fernández-MorenoRafael De Prado
Dec 6, 2016·Frontiers in Plant Science·Ricardo Alcántara-de la CruzRafael De Prado
Mar 9, 2021·Frontiers in Plant Science·José G Vázquez-GarcíaRafael De Prado
Jul 10, 2018·Journal of Agricultural and Food Chemistry·Jingbo LiStephen Powles

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.