Epstein-barr virus (EBV) in healthy carriers: Distribution of genotypes and 30 bp deletion in latent membrane protein-1 (LMP-1) oncogene

Journal of Medical Virology
Rita Mariel CorreaMaría Alejandra Picconi

Abstract

There are two types of Epstein Barr virus (EBV): EBV-1 and EBV-2, distinguished by genomic polymorphism in the genes encoding the nuclear antigens (EBNA-2, -3A, -3B, -3C). Latent membrane protein 1 (LMP-1) is an EBV protein with known oncogenic properties. Different variants had been described; among them, a 30 base pair (bp) deletion (del-LMP-1) had been reported in benign and malignant pathologies, but there is little information about its frequency in healthy populations. The aim of this study was to determine the distribution of the EBV genotypes and the 30 bp deletion frequency, in EBV healthy carriers from Argentina. Analysis of EBNA-3C and LMP-1 genes were done by polymerase chain reaction (PCR) followed by Southern blot hybridization on DNA of peripheral blood mononuclear cells (PBMCs) from blood bank donors. EBV-1 was present in 75.9% of samples, EBV-2 in 14.6%, and co-infections with both types in 6.5%. The deleted LMP-1 variant was found in 7.4% of analyzed samples, corresponding 3.2% to deleted variant alone and 4.2% to co-infections with non-deleted form. The non-deleted variant was found in 64.6% whereas in the remaining 28%, no PCR product was detected. These results showed that EBV-1 was the more prevalent type ...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis
Sep 30, 1989·Lancet·J W SixbeyL Resnick
Oct 15, 1986·Virology·U ZimberU Schneider
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J SampleE Kieff
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G Miller, M Lipman
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G MillerM Lipman
Apr 1, 1997·Journal of Medical Virology·K I FalkI Ernberg
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·J SidagisY Eizuru
May 14, 2003·Journal of Virology·Dennis M WallingPaul D Ling

❮ Previous
Next ❯

Citations

Dec 14, 2006·Journal of Pediatric Hematology/oncology·Chuen HsuehIou-Jih Hung
Oct 18, 2014·Ocular Immunology and Inflammation·Madhuravasal Krishnan JananiHajib Naraharirao Madhavan
Sep 10, 2010·Journal of Medical Virology·Mario Alejandro LorenzettiMaría Victoria Preciado
Jul 20, 2016·Journal of Medical Virology·Marco NevesHugo Sousa
Jan 1, 2012·Pathogens·Stelios Tzellos, Paul J Farrell
Feb 22, 2007·Journal of Medical Virology·Rita Mariel CorreaMaría Alejandra Picconi
May 29, 2020·Biomédica : revista del Instituto Nacional de Salud·Daniela Arturo-TerranovaAndrés Castillo
Jun 13, 2021·Leukemia·Hua WangYang Liang
May 16, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Elisabeth Puchhammer-Stöckl, Irene Görzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.