Epstein-barr virus encodes three bona fide ubiquitin-specific proteases.

Journal of Virology
Ramakrishna SompallaeMaria G Masucci

Abstract

Manipulation of the ubiquitin proteasome system (UPS) is emerging as a common theme in viral pathogenesis. Some viruses have been shown to encode functional homologs of UPS enzymes, suggesting that a systematic identification of these products may provide new insights into virus-host cell interactions. Ubiquitin-specific proteases, collectively known as deubiquitinating enzymes (DUBs), regulate the activity of the UPS by hydrolyzing ubiquitin peptide or isopeptide bonds. The prediction of viral DUBs based on sequence similarity with known enzymes is hampered by the diversity of viral genomes. In this study sequence alignments, pattern searches, and hidden Markov models were developed for the conserved C- and H-boxes of the known DUB families and used to search the open reading frames (ORFs) of Epstein-Barr virus (EBV), a large gammaherpesvirus that has been implicated in the pathogenesis of a broad spectrum of human malignancies of lymphoid and epithelial cell origin. The searches identified a limited number of EBV ORFs that contain putative DUB catalytic domains. DUB activity was confirmed by functional assays and mutation analysis for three high scoring candidates, supporting the usefulness of this bioinformatics approach in ...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J J CruteI R Lehman
Jun 9, 1995·The Journal of Biological Chemistry·S DrachevaJ J Crute
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Nov 25, 1998·Critical Reviews in Biochemistry and Molecular Biology·A D'Andrea, D Pellman
Jan 27, 1999·Bioinformatics·S R Eddy
Mar 12, 1999·Annual Review of Medicine·A L Schwartz, A Ciechanover
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A CiechanoverA L Schwartz
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
May 22, 2002·Journal of Virology·Maxim Y BalakirevJadwiga Chroboczek
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Simon S Wing
May 20, 2003·Immunological Reviews·Justine C Lee, Marcus E Peter
Jun 1, 2004·Molecular and Cellular Biology·Julia Shackelford, Joseph S Pagano
Jun 16, 2004·Current Opinion in Cell Biology·Lijun Sun, Zhijian J Chen
Jun 24, 2004·Molecular Interventions·Oonagh T Lynch, Massimo Gadina
Dec 2, 2004·Biochimica Et Biophysica Acta·Alexander Y Amerik, Mark Hochstrasser
Dec 21, 2004·Briefings in Bioinformatics·Guoqing Lu, Etsuko N Moriyama
Dec 21, 2004·Nucleic Acids Research·Amos BairochLai-Su L Yeh
Feb 8, 2005·Molecular Cell·Sebastian M B NijmanRené Bernards
Nov 23, 2005·The Journal of Experimental Medicine·Honglin ZhouVishva M Dixit
Nov 25, 2005·Journal of Virology·Christian SchliekerHidde L Ploegh
Dec 6, 2005·Cell·Sebastian M B NijmanRené Bernards
May 26, 2006·Genetics·John W Drake
Dec 6, 2006·Advances in Immunology·Joana Loureiro, Hidde L Ploegh
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anne RytkönenDavid W Holden
Apr 19, 2007·PloS One·André CaticHidde L Ploegh
May 22, 2007·Cell·George-Lucian MoldovanStefan Jentsch
Dec 15, 2007·Cell Host & Microbe·Natalia Frias-StaheliHerbert Whiting Virgin

❮ Previous
Next ❯

Citations

May 1, 2009·Nature Cell Biology·Felix Randow, Paul J Lehner
Apr 16, 2010·Journal of Virology·Shunbin Ning, Joseph S Pagano
Jul 31, 2009·Journal of Virology·Carlos M GonzálezBlossom Damania
Jun 6, 2009·Annual Review of Biochemistry·Francisca E Reyes-TurcuKeith D Wilkinson
Apr 22, 2009·PLoS Pathogens·Joy I LeeGregory A Smith
Mar 29, 2014·PLoS Pathogens·Ben A Bailey-ElkinBrian L Mark
Sep 8, 2015·Viruses·Peter Wimmer, Sabrina Schreiner
Sep 27, 2008·Biochimica Et Biophysica Acta·Mariola J Edelmann, Benedikt M Kessler
Jun 17, 2009·Cell Host & Microbe·Marisa K Isaacson, Hidde L Ploegh
Jun 20, 2017·Journal of Molecular Biology·Ben A Bailey-ElkinBrian L Mark
Jan 21, 2017·Frontiers in Microbiology·Hsiang-Hung HuangLi-Kwan Chang
Aug 28, 2020·Journal of Virology·Mengmeng ZhaoZhen Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.