PMID: 9537041Jan 1, 1980Paper

Epstein-Barr virus-induced lymphoblastoid cell lines from patients with primary immunodeficiency diseases

Scandinavian Journal of Immunology
S TsuchiyaY Ono

Abstract

Peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with Epstein-Barr virus (EBV) in an attempt to establish B lymphoblastoid cell lines (LCL). These patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with DiGeorge syndrome. Five of the patients bore no surface immunoglobulins (sIg) on their peripheral lymphocytes. LCL were established from seven of the eight patients. All the LCL established formed rosettes with EAC3 and had the ability to produce cytoplasmic immunoglobulins (cIg) of various classes. Culture supernatants concentrated up to 100-fold developed precipitin bands by Ouchterlony's method with antisera to human Ig in all the established LCL. These results suggested that both sIg-, cIg- and C3+ cells and sIg+, cIg- and C3+ cells might be the target cells for EBV and that sIg-, cIg- and C3+ cells might be the precursor cells of B lymphocytes.

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Citations

Dec 7, 2002·Critical Reviews in Oncology/hematology·Shigeru Tsuchiya
Dec 1, 1981·In Vitro·R H Kennett
Oct 1, 1983·The Journal of Pediatrics·S TsuchiyaK Tada
Jun 1, 1991·Jinrui idengaku zasshi. The Japanese journal of human genetics·M MinegishiT Konno
Jul 1, 1988·Immunological Investigations·M E Conley, J M Puck

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