Epstein-Barr virus induces an oxidative stress during the early stages of infection in B lymphocytes, epithelial, and lymphoblastoid cell lines.

Molecular and Cellular Biochemistry
Saloua LassouedHammadi Attia

Abstract

The study investigates the direct effect of Epstein-Barr virus infection on the oxidative profile of in vitro cultivated human cells. For this purpose, a panel of human EBV target cells presenting heterogeneity in their cellular and culture types (epithelial cells or lymphocytes; primary culture or continuous cell culture) was selected. These cells are purified human B lymphocytes, DG75, 293, and HepG2 cell lines. The oxidative stress was evaluated during the early stages of infection (2, 12, and 24 h) by measuring malondialdehyde, the end product of the lipid peroxidation, as well as the activities of two antioxidant enzymes: catalase and superoxide dismutase. The obtained results were compared with those of the untreated cells and the K562 cell line which has no interaction with EBV. The incubation of the different target cells with EBV induced an oxidative stress in the purified B lymphocytes, DG75, and 293, but not in HepG2 and K562. This oxidative stress was highlighted by an increase in MDA level (P < 0.05), which began 2 h after the addition of the virus and persisted after 12 and 24 h. Simultaneously, a decrease in catalase and superoxide dismutase activities was observed (P < 0.05), suggesting an alteration of the mole...Continue Reading

References

Jan 1, 1977·International Journal of Cancer. Journal International Du Cancer·H Ben-BassatG Klein
Feb 1, 1979·Cell·T DambaughE Kieff
Jan 15, 1987·International Journal of Cancer. Journal International Du Cancer·N Raab-TraubJ Pagano
Jun 1, 1987·European Journal of Immunology·M G MasucciE Klein
Jan 1, 1984·Methods in Enzymology·H Aebi
Jun 1, 1996·Methods : a Companion to Methods in Enzymology·O T G Jones, J D Wood
Jan 1, 1996·Free Radical Biology & Medicine·K B Schwarz
Feb 11, 1999·Journal of Virology·J D FingerothJ L Yates
Jun 11, 1999·The Journal of Biological Chemistry·M CarballoF Sobrino
Feb 23, 2000·Journal of Virology·M SavardJ Gosselin
Oct 9, 2002·The American Journal of Pathology·Bastiaan P van ReesG Johan A Offerhaus
Aug 12, 2003·Oncogene·Lawrence S Young, Paul G Murray
Dec 4, 2003·Biochemical Society Transactions·L BehrendR M Zwacka
May 18, 2004·Cancer Cell·Kwok Wai LoDolly P Huang
Sep 1, 2004·Biochemical and Biophysical Research Communications·Li Li, Peter E Shaw
Dec 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Francesca CerimeleJack L Arbiser
May 25, 2005·Brain Research·Tulin Otamis PriceWilliam A Banks
Dec 21, 2005·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kazuhiko Koike, Hideyuki Miyoshi
Apr 14, 2006·Neoplasia : an International Journal for Oncology Research·Angela Kwok Fung LoDolly P Huang
Sep 9, 2006·Molecular and Cellular Biochemistry·Tamara SeveriJos F van Pelt
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser

❮ Previous
Next ❯

Citations

Aug 20, 2014·The Journal of Biological Chemistry·Jonathan A TrujilloAnthony W Purcell
Oct 2, 2019·Acta Odontologica Scandinavica·Aleksandar JakovljevicJelena Milasin
Oct 8, 2019·The International Journal of Neuroscience·Tobore Onojighofia Tobore
Jun 11, 2020·Cancers·Diego GerminiYegor Vassetzky
Dec 3, 2016·Biochemistry. Biokhimii︠a︡·V A PopkovD B Zorov
Dec 17, 2008·Molecular and Cellular Biochemistry·Bochra GargouriSaloua Lassoued
Nov 10, 2009·Biological Trace Element Research·Saloua LassouedJos Van Pelt
Feb 4, 2014·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Z GajD Nowakowska
Aug 1, 2016·Current Zoology·Manrico SebastianoDavid Costantini
Jul 31, 2019·Oxidative Medicine and Cellular Longevity·Małgorzata Strycharz-DudziakMałgorzata Polz-Dacewicz
May 14, 2009·Biological Trace Element Research·Bochra GargouriAbd El Fatteh El Feki
Nov 22, 2013·Advances in Virology·Sanjeev K Anand, Suresh K Tikoo
Apr 19, 2012·Reviews in Medical Virology·Jose Antonio BogaRussel J Reiter
Dec 18, 2009·Southern Medical Journal·Bochra GargouriAbd El Fatteh El Feki
Jul 22, 2014·Free Radical Research·C G MolteniS Esposito
May 26, 2017·International Journal of Cancer. Journal International Du Cancer·Angela Kwok-Fung LoKwok-Wai Lo
Jan 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Bettina GruhneMaria G Masucci
Jun 2, 2017·International Journal of Cancer. Journal International Du Cancer·Jianmin HuYa Cao
Oct 22, 2019·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Nicholas BirkettDaniel Krewski
Jan 1, 2012·Scientifica·Lori Frappier
Oct 20, 2020·Journal of Alzheimer's Disease : JAD·Michael R DugganKamel Khalili
Jun 15, 2021·Multiple Sclerosis : Clinical and Laboratory Research·Jing WuAnna Karin Hedström
Dec 31, 2011·Seminars in Cancer Biology·Lori Frappier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.