Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice

Journal of Virology
Goran GregorovicPaul J Farrell

Abstract

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

References

Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·D P ToczyskiJ A Steitz
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Mar 4, 2010·Genome Biology·Mark D Robinson, Alicia Oshlack
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Feb 1, 2012·PLoS Pathogens·Rebecca L SkalskyBryan R Cullen
Nov 22, 2013·Journal of Virology·Rebecca L SkalskyBryan R Cullen
Nov 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shawn P FahlDavid L Wiest
Dec 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Marissa RashkovanTarik Möröy
Apr 8, 2015·Current Opinion in Virology·Cornelia GujerChristian Münz

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Citations

Jan 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·S Rubina BaglioD Michiel Pegtel
Apr 15, 2017·F1000Research·Brent A Stanfield, Micah A Luftig
Aug 21, 2018·Annual Review of Pathology·Paul J Farrell
Sep 4, 2019·Nature Reviews. Microbiology·Christian Münz
Mar 7, 2021·Pathogens·Nicole L DigginsMeaghan H Hancock
Aug 25, 2017·Current Opinion in Virology·Christian Münz
Jul 9, 2021·Frontiers in Microbiology·Christian Münz
Aug 8, 2021·Cancers·Kin Israel NotartePaul Murray

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Datasets Mentioned

BETA
PRJNA287267

Methods Mentioned

BETA
transfection
immunoprecipitation
Illumina sequencing
reverse transcription-PCR
PCR
transgenic
flow cytometry

Software Mentioned

edgeR
miRDeep2

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