EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae

PloS One
Philippa K MorrisonCaroline McG Argo

Abstract

Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop and test a semi-quantitative system (EQUIFAT) which could be applied to regional adipose tissues. Anatomically-defined, photographic images of adipose depots (omental, mesenteric, epicardial, rump) were collected from 38 animals immediately post-mortem. Images were ranked and depot-specific descriptors were developed (1 = no fat visible; 5 = excessive fat present). Nuchal-crest and ventro-abdominal-retroperitoneal adipose depot depths (cm) were transformed to categorical 5 point scores. The repeatability and reliability of EQUIFAT was independently tested by 24 observers. When half scores were permitted, inter-observer agreement was substantial (average κw: mesenteric, 0.79; omental, 0.79; rump 0.61) or moderate (average κw; epicardial, 0.60). Intra-observer repeatability was tested by 8 observers on 2 occasions. Kappa analysis indicated perfect (omental and mesenteric) and substantial agreement (epicardial and rump) between attempts...Continue Reading

References

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Citations

Jan 19, 2020·Journal of Equine Veterinary Science·Ashley L FowlerLaurie M Lawrence
Sep 18, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Serpil Ünver SaraydinDursun Saraydın
May 28, 2021·Journal of Veterinary Internal Medicine·Bettina DunkelNicola J Menzies-Gow

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Methods Mentioned

BETA
dissection

Software Mentioned

EQUIFAT
STATA

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