Equilenin-2'-deoxynucleoside adducts: analysis with nano-liquid chromatography coupled to nano-electrospray tandem mass spectrometry

Journal of Mass Spectrometry : JMS
J EmbrechtsE L Esmans

Abstract

The interaction of 4-hydroxy metabolites of estrogens with DNA leads to the formation of DNA adducts. These adducts are believed to play an important role in the incidence of breast and endometrial cancer. In order to be able to analyze these adducts in in vivo samples a method based upon the coupling of miniaturized liquid chromatography (LC) to electrospray tandem mass spectrometry (ES-MS/MS) was developed for the analysis of the adducts formed with 4-hydroxyequilenin. In vitro synthesized adducts obtained by the reaction of 4-hydroxyequilenin with the main 2'-deoxynucleosides were separated on a Hypersyl C(18) BDS nano-HPLC column (15 cm x 75 microm i.d.) at a flow-rate of 300 nl min(-1) using gradient elution with CH(3)OH--0.2% CH(3)COOH in H(2)O. The column was coupled, in combination with a column switching system, to a nano-electrospray interface. Analysis of the low- and high-resolution low-energy collision-activated dissociation product ion spectra of normal and deuterated adducts supported earlier data demonstrating equilenin to form different isomeric adducts, except with thymidine, for which no adducts were found. The nano-HPLC column-switching ES-MS system was tested for its sensitivity on a triple-quadrupole instr...Continue Reading

References

May 1, 1990·Mutation Research·J G Liehr
Nov 1, 1986·British Journal of Cancer·L A BrintonJ F Fraumeni
Sep 1, 1995·American Journal of Obstetrics and Gynecology·R A Lobo
Nov 24, 1995·Journal of Chromatography. a·W Schrader, M Linscheid
Nov 1, 1996·Carcinogenesis·H S Feigelson, B E Henderson
Apr 1, 1997·Chemical Research in Toxicology·A AkanniY J Abul-Hajj
Oct 20, 1998·Chemical Research in Toxicology·J L BoltonS Qiu
Feb 5, 2000·JAMA : the Journal of the American Medical Association·C SchairerR Hoover

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Citations

Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Yongkai SunRichard B van Breemen
Jul 19, 2005·Journal of the American Society for Mass Spectrometry·Bradley B SchneiderThomas R Covey
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Apr 18, 2008·Chemical Research in Toxicology·Shuang DingSuse Broyde
Jul 12, 2007·Journal of Separation Science·Javier Hernández-BorgesSalvatore Fanali
Sep 17, 2011·Chemistry & Biodiversity·Michael P StoneGanesh Shanmugam
Aug 21, 2010·Journal of Nucleic Acids·Gaëlle Lenglet, Marie-Hélène David-Cordonnier
Jun 8, 2001·Journal of Mass Spectrometry : JMS
Jun 17, 2003·Chemical Research in Toxicology·Shuang DingSuse Broyde

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