PMID: 6172355Dec 1, 1981Paper

Equilibrium binding constants of C3DP, a nucleic acid-binding protein isolated from human serum

Hoppe-Seyler's Zeitschrift für physiologische Chemie
C Schröder, D Weinblum

Abstract

The malignancy-associated DNA-binding protein C3DP has been isolated from normal human serum and identified by dodecyl sulfate/polyacrylamide gradient gel electrophoresis and immunodiffusion. The binding of purified C3DP to DNA and RNA has been investigated by density gradient centrifugation. The equilibrium binding constant K of C3DP binding to native human DNA was determined to be K = (2.9 +/- 0.5) x 10(5) M-1. From this value we conclude that C3DP binds non-specifically with regard to the base sequence of the DNA. K was found to be in the same order of magnitude when measured with single-stranded or double-stranded DNA of varying G + C-content, and with RNA. It varied only slightly with the pH or ionic strength of the medium, indicating that only one Na ion is released from DNA when one molecule of C3DP is bound. We conclude that ionic interactions cannot account for the stability of the C3DP-DNA complex and that therefore hydrogen bonds and hydrophobic interactions are likely to be formed.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y Kao-HuangP H von Hippel
Jan 1, 1978·The International Journal of Biochemistry·H Blüthmann
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
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Feb 28, 1970·Journal of Molecular Biology·A D RiggsS Bourgeois
May 14, 1980·Biochemical and Biophysical Research Communications·W D GardnerS O Hoch

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Citations

Feb 1, 1996·Biological Trace Element Research·S B EtcheverryE J Baran
Oct 19, 2012·Journal of Medicinal Chemistry·Robert KaplánekLinda J Roman
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K R WilliamsG L Patel

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