Equilibrium partitioning of Ficoll in composite hydrogels

Journal of Colloid and Interface Science
Kimberly B KostoWilliam M Deen

Abstract

Equilibrium partition coefficients (phi, the concentration in the gel divided by that in free solution) of fluorescein-labeled Ficolls in pure agarose and agarose-dextran composite gels were measured as a function of gel composition and Ficoll size. The four narrow fractions of Ficoll, a spherical polysaccharide, had Stokes-Einstein radii ranging from 2.7 to 5.9 nm. Gels with agarose volume fractions of 0.040 and 0.080 were studied, with dextran volume fractions (calculated as if the chain were a long fiber) up to 0.011. As expected, phi generally decreased as the Ficoll size increased (for a given gel composition) or as the amount of dextran incorporated into the gel increased (for a given agarose concentration and Ficoll size). The decrease in phi that accompanied dextran addition was predicted well by an excluded volume theory in which agarose and dextran were both treated as rigid, straight, randomly positioned and oriented fibers. Modeling dextran as a spherical coil within a fibrous agarose gel produced much less accurate predictions. The diffusional permeabilities of these gels were assessed by combining the current partitioning data with relative diffusivities (Kd, the diffusivity in the gel divided by that in free solu...Continue Reading

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Citations

Oct 23, 2009·American Journal of Physiology. Renal Physiology·William H FissellMichelle H Chen
Jan 27, 2009·Biomaterials·Emilie AllardJean-Pierre Benoit
Jan 13, 2009·Advanced Drug Delivery Reviews·Yen Cu, W Mark Saltzman
Oct 24, 2013·Biomaterials·Thomas J DurschClayton J Radke
Nov 2, 2004·Biophysical Journal·Kimberly B Kosto, William M Deen
Apr 9, 2008·Physiological Reviews·Börje HaraldssonWilliam M Deen
Nov 23, 2019·Analytical Chemistry·Alison SuAmy E Herr

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