Aug 16, 1976

Equilibrium studies on the refolding and reactivation of rabbit-muscle aldolase after acid dissociation

European Journal of Biochemistry
M EngelhardR Jaenicke


Dissociation, denaturation, and deactivation of aldolase from rabbit muscle in the acid pH range have been investigated using sedimentation analysis, fluorescence, circular dichroism, and activity tests. Under comparable experimental conditions the pH-dependent profiles of deactivation and denaturation parallel the dissociation of the enzyme. In the range of dissociation at pH4-5tetramers and monomers are in equilibrium. Intrinsic chromophores and far-ultraviolet circular dichroism suggest the transition to be a complex multistep process. At pH approximately 2.3 the enzyme is split into its fully inactive monomers which still contain some residual secondary structure. After reassociation under optimum conditions (0.2 M phosphate buffer pH 7.6, 1 mM EDTA, 0.1 mM dithiothreitol, 0 degrees C, enzyme concentration 0.4-59 mug/ml) up to 95% enzymic activity is recovered which belongs to a renatured tetrameric species indistinguishable from the native enzyme by all available biochemical and physicochemical criteria.

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Mentioned in this Paper

Phosphate buffers
Plasma Protein Binding Capacity
Protein Conformation
Spectrophotometry, Ultraviolet
Sedimentation Procedure
Circular Dichroism, Vibrational
Fructosediphosphate Aldolase

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