Equine bronchial epithelial cells differentiate into ciliated and mucus producing cells in vitro.

In Vitro Cellular & Developmental Biology. Animal
Ute E SchwabDavid G Russell

Abstract

We describe a method for creating differentiated equine bronchial epithelial cell cultures that can be used for in vitro studies including airway disease mechanisms and pathogen-host interactions. Our method is based on the culturing of human tracheobronchial epithelial cells at an air-liquid interface (ALI) in specific serum-free, hormone-supplemented medium. Bronchial epithelial cells are isolated and grown on T-Clear® insert membranes. Within 2 to 3 wk, cells differentiate into ciliated and mucus producing cells as demonstrated by confocal and electron microscopy. Furthermore, the demonstration of the two major gel-forming mucin species, Muc5ac and Muc5b, in our bronchial epithelial cell culture system validates this method for studies of respiratory tract disease of the horse.

References

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Mar 23, 2005·In Vitro Cellular & Developmental Biology. Animal·Regina K RoweAndrew Pekosz
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hirotoshi MatsuiRichard C Boucher
Feb 13, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Karine RousseauDavid J Thornton
Jul 3, 2008·In Vitro Cellular & Developmental Biology. Animal·Workineh ShibeshiFritz R Ungemach
Oct 22, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mehmet KesimerJohn K Sheehan
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L KaartinenS H Randell

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Citations

Jun 1, 2013·Veterinary Immunology and Immunopathology·Ute SchwabJulia Felippe
Jan 18, 2015·Veterinary Research·Linda FrellstedtTatiana Art
Mar 20, 2019·Scientific Reports·Jolien Van CleemputHans J Nauwynck
Apr 10, 2019·Infection and Immunity·Daniel CozensRobert L Davies

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