Equine chorionic gonadotrophin improved vascularization of feline ovarian tissue xenografted into immunosuppressed mice

Theriogenology
Luã Barbalho de MacêdoCarlos Eduardo Bezerra de Moura

Abstract

The objective of this study was to evaluate effects of eCG on vascularization and development of feline ovarian tissue xenografted to immunosuppressed mice. Feline ovarian fragments (∼1 mm3) were transplanted under the renal capsule of 20 adult, ovariectomized, C57BL/6 SCID female mice. At 45 d after transplantation, 10 mice (controls) were euthanized and the remainder given 10 IU of eCG (and sacrificed 48 h later). Transplants were recovered immediately after death, fixed, sectioned, and stained with periodic acid-Schiff (PAS). Fragment volume (Cavallieri principle) and vascularization were assessed. Mean xenotransplant volume for control and treatment groups was 0.17 ± 0.03 and 0.37 ± 0.13 mm3, respectively (P = 0.0952); vascular volume density, 30.3 ± 11.3 and 49.1 ± 8.9% (P = 0.0281); surface density, 4.1 ± 2.4 and 6.2 ± 1.7 μm-1 (P = 0.2222); and vessel total surface, 0.63 ± 0.24 μm2 and 2.28 ± 1.05 μm2 (P = 0.0079). In conclusion, eCG significantly increased vascular volume density of xenotransplanted ovarian tissue and improved its development.

References

Nov 8, 2003·Anais Da Academia Brasileira De Ciências·Carlos A Mandarim-de-Lacerda
Dec 10, 2003·Theriogenology·Monique C J ParisJill M Shaw
Jul 29, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Kazuhiro KikuchiHiroyuki Kaneko
Feb 14, 2012·Cellular & Molecular Immunology·Ryoji ItoMamoru Ito
Nov 12, 2013·Domestic Animal Endocrinology·L A FátimaP C Papa
Feb 5, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Monireh MahmoodiAbdulhussein Shahverdi
Jun 23, 2015·Microscopy Research and Technique·Carlos Eduardo Bezerra MouraPaula De Carvalho Papa
May 28, 2011·Reproductive Medicine and Biology·Mohammed Ali Abdel-GhaniHiroshi Suzuki

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