Equine class II MHC antigens: identification of two sets of epitopes using anti-human monoclonal antibodies.

Tissue Antigens
D S MonosM Kamoun

Abstract

Six mouse and 13 rat monoclonal antibodies (mAb) recognizing HLA-DR, DQ and DP antigens were used for the detection of cell surface class II MHC antigens of equine lymphocytes. The monoclonal antibodies were tested against peripheral blood lymphocytes (PBL) from a panel of thoroughbred horses, using two-color fluorescence flow cytometry. Seven of these mAbs reacted with both surface immunoglobulin positive (sIg+) and surface immunoglobulin negative (sIg-) lymphocytes. sIg+ cells stained consistently brighter than sIg- cells. The fluorescence pattern did not vary from donor to donor for each of the mAbs tested, except for SFR1-MI.2, which reacted with a variable intensity with cells from 47 of 53 horses tested. Immunoprecipitation with mAb SFR1-MI.2 and analysis by two-dimensional electrophoresis demonstrated the presence of light and heavy chains equivalent to HLA class II alpha and beta chains. Antibody N297 (DQ specific), previously shown to react with an epitope expressed on human B cells but not on mitogen-induced T cells, reacted only with sIg+ cells in 42 of 53 horses tested. The lack of staining of horses sIg- cells with N297 may be due to a low or lack of expression of this determinant on these cells or to a weak cross-...Continue Reading

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Citations

Jan 1, 1993·Veterinary Immunology and Immunopathology·D P LunnW P Duffus
Sep 1, 1995·Veterinary Immunology and Immunopathology·P DirscherlA Ziesenis
Jul 1, 1994·Veterinary Immunology and Immunopathology·J KyddM A Holmes
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Jan 1, 1993·The British Veterinary Journal·D P Lunn
Jun 25, 1998·Veterinary Immunology and Immunopathology·D P LunnK E Vagnoni
Nov 1, 1990·Tissue Antigens·B A RideoutN C Pedersen
Nov 29, 2008·Veterinary Microbiology·Isabel Fidalgo-CarvalhoRonald C Montelaro
Dec 5, 1998·Journal of Comparative Pathology·C M KalsowD P Lunn
Aug 19, 2007·Veterinary Immunology and Immunopathology·Sherif Ibrahim, Falko Steinbach
Mar 1, 1993·Zeitschrift für Ernährungswissenschaft·L AustJ Proll

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