Equine cloning: in vitro and in vivo development of aggregated embryos

Biology of Reproduction
Andrés GambiniDaniel F Salamone

Abstract

The production of cloned equine embryos remains highly inefficient. Embryo aggregation has not yet been tested in the equine, and it might represent an interesting strategy to improve embryo development. This study evaluated the effect of cloned embryo aggregation on in vitro and in vivo equine embryo development. Zona-free reconstructed embryos were individually cultured in microwells (nonaggregated group) or as 2- or 3-embryo aggregates (aggregated groups). For in vitro development, they were cultured until blastocyst stage and then either fixed for Oct-4 immunocytochemical staining or maintained in in vitro culture where blastocyst expansion was measured daily until Day 17 or the day on which they collapsed. For in vivo assays, Day 7-8 blastocysts were transferred to synchronized mares and resultant vesicles, and cloned embryos were measured by ultrasonography. Embryo aggregation improved blastocyst rates on a per well basis, and aggregation did not imply additional oocytes to obtain blastocysts. Embryo aggregation improved embryo quality, nevertheless it did not affect Day 8 and Day 16 blastocyst Oct-4 expression patterns. Equine cloned blastocysts expanded and increased their cell numbers when they were maintained in in vi...Continue Reading

References

Sep 1, 1988·Biology of Reproduction·K J McDowellC J Wilcox
Sep 1, 1972·Journal of Reproduction and Fertility·H R TervitL E Rowson
May 20, 1998·Molecular Reproduction and Development·T T PeuraA O Trounson
Feb 5, 2000·Molecular Reproduction and Development·G VajtaH Callesen
May 23, 2002·Genes & Development·Michele BoianiK John McLaughlin
May 31, 2003·Science·Gordon L WoodsBarry J Pate
Aug 9, 2003·Nature·Cesare GalliGiovanna Lazzari
Jul 23, 2004·Cloning and Stem Cells·P Chavatte-PalmerJ-P Mialot
Jul 24, 2004·Animal Reproduction Science·Sigrid Eckardt, K John McLaughlin
Jan 1, 2005·Journal of the American Veterinary Medical Association·Dirk K VanderwallKenneth L White
Apr 20, 2005·Molecular and Cellular Endocrinology·Hanne G PedersenTorben Greve
Jun 30, 2005·Zygote : the Biology of Gametes and Early Embryos·P Chrenek, A V Makarevich
Jun 30, 2005·Theriogenology·Katrin Hinrichs
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Irina LagutinaCesare Galli
Aug 1, 1982·Theriogenology·O J Ginther, R H Douglas
Oct 1, 1990·Theriogenology·K HinrichsK M Ebert
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K HinrichsD D Varner
Apr 19, 2007·Molecular Reproduction and Development·Sang-Goo LeeChang-Kyu Lee
Apr 21, 2007·The Journal of Reproduction and Development·Ralf PoehlandSanjay Bhojwani
Jun 15, 2007·Theriogenology·M A MiglinoA M Carter
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·K HinrichsD L Hartman
Jan 16, 2008·Molecular Reproduction and Development·Wenli ZhouIrina Polejaeva
Jun 16, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Y H ChoiK Hinrichs
May 4, 2010·Journal of the American Veterinary Medical Association·Aime K JohnsonKatrin Hinrichs

❮ Previous
Next ❯

Citations

Sep 4, 2015·Reproduction in Domestic Animals = Zuchthygiene·L N MoroD F Salamone
Feb 20, 2016·PloS One·Carla Paola BuemoDaniel Felipe Salamone
Nov 3, 2016·Zygote : the Biology of Gametes and Early Embryos·Dong-Kyung LeeChang-Kyu Lee
Dec 19, 2012·Reproduction, Fertility, and Development·Katrin Hinrichs
Nov 7, 2019·Reproduction in Domestic Animals = Zuchthygiene·Marta Dordas-PerpinyàJean-François Bruyas
Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·Katrin Hinrichs
Oct 5, 2019·Reproduction, Fertility, and Development·Thiago V Damasceno TeixeiraAndrés Gambini
Nov 21, 2019·Reproduction, Fertility, and Development·María Belén RodríguezDaniel F Salamone
Mar 6, 2021·Journal of Equine Veterinary Science·Erin HiseyStuart A Meyers
Mar 31, 2021·Journal of Equine Veterinary Science·Erin A HiseyStuart Meyers

❮ Previous
Next ❯

Methods Mentioned

BETA
scraping
biopsies
light microscopy

Software Mentioned

EZ
Statistix
C1

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.