Equine herpesvirus 1 mutants devoid of glycoprotein B or M are apathogenic for mice but induce protection against challenge infection

Virology
A NeubauerNikolaus Osterrieder

Abstract

Equine herpesvirus 1 (EHV-1) mutants devoid of the open reading frames (ORFs) of either glycoprotein (g) B or M were constructed and tested for their immunogenic potential in a murine model of EHV-1 infection. The mutant viruses were engineered using the virulent EHV-1 strain RacL11 or the modified live vaccine strain RacH by inserting the Escherichia coli LacZ gene into the viral ORFs. RacL11-infected mice showed signs typical of an EHV-1 infection, whereas mice infected with the EHV-1 gB- or gM-negative mutants or with RacH did not develop disease. No difference in the pathogenic potential of RacL11 gB- and gM-negative viruses was observed after application of either phenotypically completed or negative viruses. However, revertant RacL11 viruses in which the gB or gM gene had been restored caused EHV-1-related symptoms that were indistinguishable from those induced by RacL11. Mice that had been immunized with phenotypically negative gB- and gM-deficient EHV-1 were challenged with the RacL11 virus 25 days after immunization. Mock-immunized mice developed EHV-1 disease and high virus loads in their lungs were observed. In contrast, mice developed not exhibit EHV-1-caused disease. It was concluded (i) that deletion of either gB ...Continue Reading

References

Jul 1, 1992·Virology·E A TelfordA J Davison
Dec 1, 1989·Virology·D C SullivanD J O'Callaghan
Jul 1, 1995·Journal of Virological Methods·P A van WoenselN Visser
Mar 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·P H HübertN Osterrieder
May 26, 1997·Virology·N OsterriederJ D Baines

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Citations

Nov 9, 2000·Journal of Virology·C M Lake, L M Hutt-Fletcher
Feb 7, 2001·Journal of Virology·A R BrackT C Mettenleiter
Apr 14, 2005·Journal of Virology·Sascha TrappNikolaus Osterrieder
Feb 3, 2006·Expert Review of Vaccines·Cristina T RosasNikolaus Osterrieder
Jun 7, 2007·The Journal of Veterinary Medical Science·Yasser M GhanemHideto Fukushi

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