Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates

Stem Cell Reports
Mattia QuattrocelliMaurilio Sampaolesi

Abstract

Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage.

References

Aug 24, 2007·Reproduction, Fertility, and Development·R Tayfur Tecirlioglu, Alan O Trounson
Apr 1, 2008·The Veterinary Journal·Dominique-M Votion, Didier Serteyn
Apr 7, 2009·Cell·Shinya Yamanaka
Feb 23, 2011·The Journal of Pathology·Mattia QuattrocelliMaurilio Sampaolesi
Jun 22, 2012·The Veterinary Journal·Jan H SpaasGerlinde R Van de Walle
Aug 18, 2012·Stem Cells and Development·Amandine BretonFrancesc Xavier Donadeu
May 4, 2013·The Veterinary Journal·P René van Weeren, Leo B Jeffcott
Jun 20, 2013·The Veterinary Journal·Lauren V SchnabelKarl M Nobert
Oct 17, 2013·The Veterinary Journal·Alberto Cebrian-SerranoAndras Dinnyes
Jan 15, 2014·Stem Cell Research & Therapy·Catharina De SchauwerGerlinde R Van de Walle
Feb 20, 2014·Stem Cells and Development·Ruchi SharmaFrancesc Xavier Donadeu
Aug 2, 2014·Journal of the American College of Cardiology·Veronica Sanchez-FreireJoseph C Wu
Oct 10, 2014·Cell Death & Disease·M QuattrocelliM Sampaolesi
Apr 25, 2015·Nature Reviews. Molecular Cell Biology·Margaret A GoodellNoah Shroyer

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Citations

Jul 6, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Y Z PatersonD J Guest
Sep 13, 2018·Cellular Reprogramming·Karin R AmilonF Xavier Donadeu
Oct 1, 2016·Dementia & Neuropsychologia·Mônica Santoro HaddadIrina Kerkis
Jun 7, 2019·Journal of Applied Toxicology : JAT·Justyna AugustyniakFrancesca Caloni
Jun 27, 2020·Stem Cell Research & Therapy·Fabiana Fernandes BressanFlávio Vieira Meirelles
Sep 17, 2019·World Journal of Stem Cells·Laís Vicari de Figueiredo PessôaKristine Karla Freude
Aug 6, 2020·International Journal of Molecular Sciences·Daniela Gois BeghiniAndrea Henriques-Pons
Jul 31, 2019·Stem Cells and Development·Xiaoqiang CongJiesi Luo
Apr 25, 2018·Biology Open·Arabella BairdDeborah J Guest
Oct 25, 2020·Journal of Animal Science·Yue SuYoung Tang
Jun 9, 2020·Mechanisms of Ageing and Development·Dovydas SirvinskasFrancesco Neri

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Methods Mentioned

BETA
biopsies
biopsy
PCR
Assay

Clinical Trials Mentioned

NCT01879046

Software Mentioned

qBase
Sample Size Calculator

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