Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal

Archives of Virology
Andreia Elisa CursinoErna G Kroon

Abstract

Equine infectious anemia (EIA) has a worldwide distribution, and is widespread in Brazil. The Brazilian Pantanal presents with high prevalence comprising equine performance and indirectly the livestock industry, since the horses are used for cattle management. Although EIA is routinely diagnosed by the agar gel immunodiffusion test (AGID), this serological assay has some limitations, so PCR-based detection methods have the potential to overcome these limitations and act as complementary tests to those currently used. Considering the limited number of equine infectious anemia virus (EIAV) sequences which are available in public databases and the great genome variability, studies of EIAV detection and characterization molecular remain important. In this study we detected EIAV proviral DNA from 23 peripheral blood mononuclear cell (PBMCs) samples of naturally infected horses from Brazilian Pantanal using a semi-nested-PCR (sn-PCR). The serological profile of the animals was also evaluated by AGID and ELISA for gp90 and p26. Furthermore, the EIAV PCR amplified DNA was sequenced and phylogenetically analyzed. Here we describe the first EIAV sequences of the 5' LTR of the tat gene in naturally infected horses from Brazil, which prese...Continue Reading

References

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Citations

Dec 8, 2020·Transboundary and Emerging Diseases·Andreia Elisa CursinoErna Geessien Kroon
Mar 14, 2021·Preventive Veterinary Medicine·Gustavo MachadoEduardo de Freitas Costa
Apr 3, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·César I Romo-SáenzRicardo Gomez-Flores
Apr 17, 2021·BMC Veterinary Research·Diana LupulovicAymeric Hans
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Alexandra Knox, Travis Beddoe

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Datasets Mentioned

BETA
DV117
DLV18-8

Methods Mentioned

BETA
enzyme linked immunosorbent assays
ELISA
PCR
PCRs
electrophoresis

Software Mentioned

FigTree
nBLAST
Mega
nBLAST )
OligoAnalyzer
Basic Local Alignment Search Tool ( BLAST )
Basic Local Alignment Search Tool program ( BLAST )
SeqTrace
Clustal W
jModel

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