Equine laminitis: membrane type matrix metalloproteinase-1 (MMP-14) is involved in acute phase onset

Equine Veterinary Journal
M Kyaw-TannerC C Pollitt

Abstract

Enzymatic separation at the hoof lamellar dermal-epidermal interface may play a role in the development of laminitis and characterising and locating matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitors of MMPs or TIMPs) in lamellar tissues may further understanding of pathogenesis. To clone and sequence the cDNA encoding lamellar MMP-14 and TIMP-2, and quantify their transcription in normal and laminitic tissue; and to develop antibody to locate MMP-14 in lamellar tissues. Tissue samples were obtained from an oligofructose induced model of laminitis. Total RNA was isolated, amplified by RT-PCR, cloned into a vector and sequenced. Real-time PCR was used to quantify MMP-14 and TIMP-2 expression. Rabbit anti-equine MMP-14 antibody was developed to analyse MMP-14 proteins from hoof tissues. Immunohistochemistry detected MMP-14 in the cytoplasm of normal lamellar basal and parabasal cells in close proximity to the lamellar basement membrane. In laminitis affected tissue MMP-14 immunostaining was depleted in lamellar basal cells. Quantitative real-time PCR showed MMP-14 and TIMP-2 expression significantly (P<0.05) elevated and lowered respectively in laminitis affected tissues. MMP-14, located in the cytoplasm of ...Continue Reading

References

Jan 1, 1993·Physiological Reviews·P Mignatti, D B Rifkin
Jan 1, 1996·Equine Veterinary Journal·C C Pollitt
Jan 17, 1996·International Journal of Cancer. Journal International Du Cancer·C GillesJ M Foidart
May 20, 1998·The Veterinary Record·P J JohnsonN T Messer
Oct 3, 1998·Equine Veterinary Journal·P D CleggS D Carter
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 3, 2003·Trends in Cell Biology·Susann Schenk, Vito Quaranta
May 19, 2004·Equine Veterinary Journal·M Daradka, C C Pollitt
May 19, 2004·Equine Veterinary Journal·K R French, C C Pollitt
May 19, 2004·Equine Veterinary Journal·A W van Eps, C C Pollitt
May 19, 2006·Equine Veterinary Journal·A W van Eps, C C Pollitt

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Citations

Jul 12, 2014·European Journal of Pharmacology·Rock Allister LapoMohamed Yassine Mallem
Apr 5, 2011·The Veterinary Journal·Carlos E Medina-TorresAli Mobasheri
Nov 9, 2010·The Veterinary Clinics of North America. Equine Practice·Simon N CollinsKaspar Matiasek
Apr 13, 2010·The Veterinary Clinics of North America. Equine Practice·Andrew W van Eps
Apr 13, 2010·The Veterinary Clinics of North America. Equine Practice·Micheal L Steward
Apr 13, 2010·The Veterinary Clinics of North America. Equine Practice·Christopher C Pollitt
Apr 13, 2010·The Veterinary Clinics of North America. Equine Practice·Susan C Eades
Apr 13, 2010·The Veterinary Clinics of North America. Equine Practice·Christopher C Pollitt, Michelle B Visser
Nov 22, 2008·The Veterinary Journal·Abigail L ClutterbuckAli Mobasheri
Nov 26, 2009·Equine Veterinary Journal·A R NourianC C Pollitt
Aug 6, 2008·Equine Veterinary Journal·I A Silver
Oct 31, 2012·Equine Veterinary Journal·L M Katz, S R Bailey
Apr 19, 2011·Equine Veterinary Journal·M B Visser, C C Pollitt
Jun 24, 2011·Equine Veterinary Journal·M B Visser, C C Pollitt
Nov 4, 2016·Anatomia, histologia, embryologia·K HarfoushE Betti
Feb 22, 2011·Veterinary Immunology and Immunopathology·M A de LaatC C Pollitt
Jul 23, 2019·Veterinary Dermatology·Tanja SteimerRalf S Mueller
Nov 20, 2019·Toxicologic Pathology·Qingqiu Yang, Mandi J Lopez
Feb 3, 2012·Journal of the Science of Food and Agriculture·Annette C LonglandPatricia A Harris

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