ER-60, a chaperone with thiol-dependent reductase activity involved in MHC class I assembly

The EMBO Journal
J A LindquistG J Hämmerling

Abstract

The assembly of newly synthesized MHC class I molecules within the endoplasmic reticulum and their association with the transporter associated with antigen processing (TAP) is a process involving the chaperones calnexin and calreticulin. Using peptide mapping by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to identify a new component, we now introduce a third molecular chaperone, the thiol-dependent reductase ER-60 (ERp57/GRP58/ERp61/HIP-70/Q2), into this process. ER-60 is found in MHC class I heavy chain complexes with calnexin that are generated early during the MHC class I assembly pathway. The thiol reductase activity of ER-60 raises the possibility that ER-60 is involved in the disulfide bond formation within heavy chains. In addition, ER-60 is part of the late assembly complexes consisting of MHC class I, tapasin, TAP, calreticulin and calnexin. In a beta2-microglobulin (beta2m)-negative mouse cell line, S3, ER-60-calnexin-heavy chain complexes are shown to bind to TAP, suggesting that beta2m is not required for the association of MHC class I heavy chains with TAP.

References

Jun 1, 1995·European Journal of Immunology·M F BachmannR M Zinkernagel
Aug 1, 1994·Trends in Biochemical Sciences·R B FreedmanM F Tuite
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Nov 1, 1993·Electrophoresis·J E CelisM Nielsen
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E van Leeuwen, K P Kearse
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Feb 1, 1997·Current Opinion in Immunology·J O KoopmannF Momburg
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·S LiP Wang
Aug 1, 1997·Immunology Today·T Elliott

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Citations

Jun 26, 1999·European Journal of Immunology·N BangiaP Cresswell
Apr 12, 2002·Journal of Cellular Biochemistry·Sabina CoppariCarlo Turano
May 20, 2006·Pflügers Archiv : European journal of physiology·Meike Herget, Robert Tampé
Nov 1, 2007·Journal of Bioenergetics and Biomembranes·Daphne Nikles, Robert Tampé
Jun 27, 2002·Biochemical and Biophysical Research Communications·Caterina GrilloFabio Altieri
Jun 4, 1999·Progress in Biophysics and Molecular Biology·K A Resing, N G Ahn
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Jun 1, 2002·Human Immunology·William H HildebrandJoyce C Solheim
Feb 27, 1999·Current Opinion in Immunology·P M van Endert
Feb 27, 1999·Current Opinion in Immunology·H SchildH G Rammensee
Nov 18, 1998·Current Opinion in Structural Biology·E S Trombetta, A Helenius
Jun 6, 2002·Immunology·Antony N AntoniouSimon J Powis
May 22, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S V Fromm, R Ehrlich
Jun 13, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Gary G GuoPravin B Sehgal
Sep 19, 2003·Antioxidants & Redox Signaling·Antony N Antoniou, Simon J Powis
May 18, 2007·Molecular Biology of the Cell·Marcel van LithAdam M Benham
Jul 10, 1999·Immunological Reviews·H HengelU H Koszinowski
Jul 10, 1999·Immunological Reviews·F M BrodskyE M Bennett
Jan 13, 2000·Immunological Reviews·G J HämmerlingH Kropshofer
Jan 13, 2000·Immunological Reviews·J C Solheim
Jan 13, 2000·Immunological Reviews·P CresswellG Diedrich
Nov 12, 2003·Journal of Virology·Derek E DimcheffJohn L Portis
Dec 28, 1999·Annual Review of Cell and Developmental Biology·J W Yewdell, J R Bennink

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