ER assembly of SNARE complexes mediating formation of partitioning membrane in Arabidopsis cytokinesis

ELife
Matthias KarnahlGerd Jürgens

Abstract

Intracellular membrane fusion mediates diverse processes including cell growth, division and communication. Fusion involves complex formation between SNARE proteins anchored to adjacent membranes. How and in what form interacting SNARE proteins reach their sites of action is virtually unknown. We have addressed this problem in the context of plant cell division in which a large number of TGN-derived membrane vesicles fuse with one another to form the partitioning membrane. Blocking vesicle formation at the TGN revealed cis-SNARE complexes. These inactive cytokinetic SNARE complexes were already assembled at the endoplasmic reticulum and, after passage through Golgi/TGN to the cell division plane, transformed into fusogenic SNARE complexes. This mode of trafficking might ensure delivery of large stoichiometric quantities of SNARE proteins required for forming the partitioning membrane in the narrow time frame of plant cytokinesis. Such long-distance trafficking of inactive SNARE complexes would also facilitate directional growth processes during cell differentiation.

References

Feb 12, 1998·The Journal of Cell Biology·M H LauberG Jürgens
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jun 18, 2002·Plant Physiology·Haiyan ZhengNatasha V Raikhel
Feb 26, 2003·Cell·Reinhard JahnThomas C Südhof
May 10, 2003·Nature Cell Biology·Isabel MüllerGerd Jürgens
Nov 13, 2007·Current Biology : CB·Ilka ReichardtGerd Jürgens
Feb 15, 2008·Nature·Chian KwonPaul Schulze-Lefert
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Josep Rizo, Thomas C Südhof
Mar 22, 2013·Molecular Biology of the Cell·Farid El KasmiGerd Jürgens
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Misoon ParkGerd Jürgens
Dec 10, 2014·Developmental Cell·Beata Mierzwa, Daniel W Gerlich
Sep 13, 2015·Critical Reviews in Biochemistry and Molecular Biology·Kazuhisa Nakayama
Nov 4, 2015·Seminars in Cell & Developmental Biology·Sabine Müller, Gerd Jürgens

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Citations

Feb 24, 2019·Annual Review of Plant Biology·Pantelis Livanos, Sabine Müller
Apr 21, 2020·Frontiers in Plant Science·Rik HuismanErik Limpens
Sep 16, 2021·The Plant Cell·Alice Y CheungDolf Weijers
Oct 7, 2021·The Plant Cell·Ying Gu, Carolyn G Rasmussen

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Methods Mentioned

BETA
co-immunoprecipitation
immunoprecipitation
transgenic
co-immunoprecipitation assay
PCR
chemical treatment

Software Mentioned

Adobe Illustrator
ImageJ
Fiji
Adobe Photoshop
Fiji ImageJ

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