PMID: 8613681Mar 1, 1996Paper

ERCC1/ERCC4 5'-endonuclease activity as a determinant of hypoxic cell radiosensitivity

International Journal of Radiation Biology
D MurrayE Rosenberg

Abstract

In this study, the relationships between cellular oxygen enhancement ratios (OER) and nucleotide excision repair capability were examined using the UV20 mutant cell line (which has a defective ERCC1 gene). Using a clonogenic survival assay, the OER for the killing of wild-type AA8 cells was 3.2 +/- 0.1, whereas that for UV20 cells was only 2.35 +/- 0.05; the decreased OER of UV20 cells was the result of their significantly greater radiosensitivity relative to wild-type cells under hypoxic conditions. In AA8 cells, hypoxia protected against DNA double-strand break (dsb) induction (determined by pulsed-field gel electrophoresis) by a factor 3.5 +/- 0.3; i.e. to a similar extent that it modulated cell killing. However, this correlation was not apparent in UV20 cells, where hypoxia protected against dsb induction to a similar extent as in wild-type cells (approximately 3.2-fold). Stably transfected UV20 cells over-expressing a full-length ERCC1 cDNA clone displayed a normal OER (3.5 +/- 0.1) in addition to wild-type resistance to UV light. Our data suggest that the hypoxic radiosensitivity of UV20 cells is a direct result of their ERCC1 deficiency and reflects their inability to process some type of DNA damage (not dsbs) that is in...Continue Reading

References

Aug 16, 1977·Biochimica Et Biophysica Acta·A J Fornace, J B Little
Jan 1, 1992·International Journal of Radiation Biology·S J Whitaker, T J McMillan
Jun 1, 1988·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·D MurrayS C Vanankeren
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M C Paterson, R B Setlow
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L H ThompsonD A Glaser
Oct 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M EdgrenA Larsson
May 1, 1980·Somatic Cell Genetics·L H ThompsonK Brookman
Dec 23, 1994·Science·A Sancar
May 1, 1993·International Journal of Radiation Biology·D T GoodheadR Cox
Jun 1, 1993·Trends in Genetics : TIG·J H Hoeijmakers

❮ Previous
Next ❯

Citations

Jun 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L HampsonI N Hampson
Feb 15, 2008·Nature Reviews. Cancer·Robert G Bristow, Richard P Hill
May 5, 2011·Physics in Medicine and Biology·Tatiana Wenzl, Jan J Wilkens
Aug 12, 2005·The Journal of Biological Chemistry·Sharon BarkerDavid Murray
Oct 7, 2003·American Journal of Clinical Oncology·David MurrayM Joan Allalunis-Turner
Aug 10, 2011·Radiation Research·Robert D StewartDavid J Carlson
Aug 10, 2000·Radiation Research·D MurrayM J Allalunis-Turner
May 30, 2009·International Journal of Radiation Biology·Ping-Kun ZhouJing An
Jan 29, 2017·Cellular and Molecular Life Sciences : CMLS·Janin LehmannSteffen Emmert
Oct 4, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ranee MehraBarbara Burtness
Mar 13, 2001·Biochemical and Biophysical Research Communications·M Sook KimK W Kim
Oct 2, 2001·Pharmacology & Therapeutics·M LeszczynieckaP B Fisher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.