Erectile tissue molecular alterations with aging: differential activation of the p42/44 MAP Kinase pathway.

Age
Angela CastelaCarla Costa

Abstract

Erectile dysfunction (ED) is a common problem in aged men; however, the molecular events involved in aging ED remain unclear. To better characterize the effects of aging in the penis, we evaluated cavernosal tissue remodeling capability and the downstream activation of the intracellular signaling mediator mitogen-activated protein p42/44 kinase (p42/44 MAPK). We used male Wistar rats, which were divided in groups of 2, 6, 12, 18, and 24 months old. Penile tissues were harvested and processed for protein isolation and immunohistochemical analysis. Cavernosal viability was assessed by TUNEL assay, and proliferation was analyzed by immunohistochemical detection of proliferating cell nuclear antigen (PCNA). Immunolocalization of the activated form of p42/44 MAPK was evaluated by immunofluorescence, and changes in its phosphorylation status were quantified by western blotting. p42/44 phosphorylation profile was also assessed in situ in human young and elderly cavernosal samples. With the advancement of age, experimental cavernosal tissue remodeling was affected by an age-dependent unbalance between the rate of apoptosis and proliferation, in all erectile components. Moreover, this turnover alteration was accompanied by significant m...Continue Reading

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Citations

Jan 18, 2019·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Kyungtae KoDae Yul Yang
Aug 19, 2017·Canadian Journal of Physiology and Pharmacology·Jaqueline J MunizCarlos R Tirapelli
Mar 12, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Luisa CostanzoClaudio Pedone
Jun 8, 2017·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·Ecem KayaSerap Gur

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