ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis.

PloS One
Caroline S MoffatMarc R Knight

Abstract

The ethylene response factor (ERF) family in Arabidopsis thaliana comprises 122 members in 12 groups, yet the biological functions of the majority remain unknown. Of the group IX ERFs, the IXc subgroup has been studied the most, and includes ERF1, ERF14 and ORA59, which play roles in plant innate immunity. Here we investigate the biological functions of two members of the less studied IXb subgroup: ERF5 and ERF6. In order to identify potential targets of these transcription factors, microarray analyses were performed on plants constitutively expressing either ERF5 or ERF6. Expression of defense genes, JA/Et-responsive genes and genes containing the GCC box promoter motif were significantly upregulated in both ERF5 and ERF6 transgenic plants, suggesting that ERF5 and ERF6 may act as positive regulators of JA-mediated defense and potentially overlap in their function. Since defense against necrotrophic pathogens is generally mediated through JA/Et-signalling, resistance against the fungal necrotroph Botrytis cinerea was examined. Constitutive expression of ERF5 or ERF6 resulted in significantly increased resistance. Although no significant difference in susceptibility to B. cinerea was observed in either erf5 or erf6 mutants, the...Continue Reading

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Citations

Jun 12, 2014·BMC Genomics·Stephanie M JohnsonMarc R Knight
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Datasets Mentioned

BETA
E-MTAB-436
E-MTAB-435
1289228646825
ME00341

Methods Mentioned

BETA
transgenic
PCR
genotyping
UV-C
Chips

Software Mentioned

BASE
FatiGO
Applied
ImageJ
BlueFuse for Microarrays
Primer Express
Biosystems Real - Time PCR Systems
ImageViewer
eFP Browser

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